The Phospho-CaMK2A (T286) Polyclonal Antibody (CABP0255) is a valuable tool for researchers studying CaMK2A, a key protein involved in various cellular processes, including synaptic plasticity and memory formation. This antibody, produced in rabbits, exhibits high specificity towards human samples and is validated for use in Western blot applications. It specifically recognizes the phosphorylated form of CaMK2A at threonine 286, allowing for the detection and analysis of this important signaling molecule in different cell types.CaMK2A, a member of the calcium/calmodulin-dependent protein kinase family, is known to play a crucial role in neuronal functions and synaptic plasticity. Phosphorylation of CaMK2A at threonine 286 is associated with its activation and involvement in various cellular pathways.
Therefore, understanding the regulation of this phosphorylation site is essential for unraveling the complex mechanisms underlying neuronal signaling and memory processes.Research on CaMK2A and its phosphorylation at threonine 286 is relevant in the fields of neuroscience, neurobiology, and memory research. By utilizing the Phospho-CaMK2A (T286) Polyclonal Antibody (CABP0255), researchers can explore the functional implications of CaMK2A phosphorylation, leading to a better understanding of its role in neuronal signaling and potential implications in neurological disorders.
Product Name:
Phospho-Camk2a-T286 Rabbit Polyclonal Antibody
SKU:
CABP0255
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around T286 of human Camk2a (NP_741960.1).
The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Several transcript variants encoding distinct isoforms have been identified for this gene.
Purification Method:
Affinity purification
Gene ID:
815
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of Mouse brain, using Phospho-Camk2a-T286 antibody (CABP0255) at 1:4000 dilution.Mouse brain cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
