The Phospho-CaMK2 (T286) Monoclonal Antibody is a vital tool for researchers studying CaMK2, a key regulator of neuronal signaling and synaptic plasticity. This antibody, produced through monoclonal antibody technology, is highly specific to the phosphorylated form of CaMK2 at threonine 286 and has been validated for use in various applications, including Western blotting and immunofluorescence.CaMK2 is a serine/threonine protein kinase that plays a crucial role in multiple cellular processes, including learning and memory, neuronal development, and synaptic transmission. Phosphorylation of CaMK2 at threonine 286 is known to regulate its kinase activity and subcellular localization, making it a critical event in neuronal signaling pathways.
This antibody specifically targets this phosphorylation site, allowing researchers to study the activation and function of CaMK2 in different cell types and experimental conditions.Understanding the regulation of CaMK2 phosphorylation at threonine 286 is essential for unraveling the molecular mechanisms underlying synaptic plasticity and neuronal communication. By using this monoclonal antibody, researchers can gain valuable insights into the role of CaMK2 in neurological disorders, such as Alzheimer's disease, epilepsy, and schizophrenia, and potentially identify new therapeutic targets for these conditions. Explore the intricate world of neuronal signaling with the Phospho-CaMK2 (T286) Monoclonal Antibody.
Product Name:
Phospho-Camk2-T286 Monoclonal Antibody
SKU:
CABP1386
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around T286 of human Camk2.
The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2 )/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Several transcript variants encoding distinct isoforms have been identified for this gene.
Purification Method:
Affinity purification
Gene ID:
816/817/818/815
Clone Number:
ARC57709
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using Phospho-Camk2a-T286 antibody (CABP1386) at 1:5000 dilution.Mouse brain cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
