The Phospho c-Myc (T58/S62) Rabbit Monoclonal Antibody (CABP0988) is a key tool for researchers studying the c-Myc protein, a well-known regulator of cell growth and proliferation. This antibody, developed using rabbit monoclonal technology, specifically targets the phosphorylation sites T58 and S62 on the c-Myc protein.This antibody is highly reactive with human samples, making it an excellent choice for experiments involving c-Myc signaling pathways. It has been validated for use in Western blot applications, enabling researchers to detect and analyze phosphorylated c-Myc in various cell types. The phosphorylation of c-Myc at T58 and S62 is known to have important implications in cancer research, as dysregulation of c-Myc signaling is commonly observed in many tumor types.
By targeting these specific phosphorylation sites, researchers can better understand the role of c-Myc in cancer progression and potentially identify new therapeutic targets.Overall, the Phospho c-Myc (T58/S62) Rabbit Monoclonal Antibody (CABP0988) is a valuable tool for researchers investigating the functions of c-Myc in cancer biology and beyond. Its high specificity and sensitivity make it an essential component for studies aiming to unravel the complex mechanisms underlying c-Myc signaling pathways.
Product Name:
Phospho-c-Myc-T58/S62 Rabbit Monoclonal Antibody
SKU:
CABP0988
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Rat
Immunogen:
A synthetic phosphorylated peptide around T58 & S62 of human c-Myc (P01106).
Sequence:
TPPL S
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
MRTL; MYCC; c-Myc; bHLHe39; Phospho-c-Myc-T58/S62
Positive Sample:
C6
Conjugate:
Unconjugated
Cellular Localization:
Nucleus, nucleolus, nucleoplasm.
Calculated MW:
51kDa
Observed MW:
51kDa
This gene is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini.
Purification Method:
Affinity purification
Gene ID:
4609
Clone Number:
ARC1532
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of C6 cells, using Phospho-c-Myc-T58/S62 Rabbit mAb (CABP0988) at 1:1000 dilution.C6 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
