The Phospho c-Myc (T58) Rabbit Monoclonal Antibody (CABP0990) is a specialized antibody designed for research involving the phosphorylated form of the c-Myc protein. This antibody, raised in rabbits, is highly reactive and specific to the T58 phosphorylation site of the c-Myc protein in human samples. It is validated for use in various applications, such as Western blotting, immunofluorescence, and immunohistochemistry.The c-Myc protein is a transcription factor that regulates cell growth and proliferation, making it a key player in cancer development and progression. Phosphorylation at the T58 site is a critical post-translational modification that can regulate the stability and activity of c-Myc, impacting its function in controlling cell growth and survival.
The Phospho c-Myc (T58) Rabbit Monoclonal Antibody enables researchers to study the phosphorylation status of c-Myc and its implications in cancer biology.By utilizing this antibody in their research, scientists can gain valuable insights into the role of phospho c-Myc (T58) in cancer signaling pathways, tumor progression, and potential therapeutic targets. This antibody is an essential tool for studies in molecular biology, cancer research, and drug development focused on targeting the c-Myc pathway.
Product Name:
Phospho-c-Myc-T58 Rabbit Monoclonal Antibody
SKU:
CABP0990
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around T58 of human c-Myc (P01106).
Sequence:
LPTP P
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Synonyms:
MRTL; MYCC; c-Myc; bHLHe39; Phospho-c-Myc-T58
Positive Sample:
HeLa
Conjugate:
Unconjugated
Cellular Localization:
Nucleus, Nucleolus, Nucleoplasm.
Calculated MW:
51kDa
Observed MW:
57kDa
This gene is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini.
Purification Method:
Affinity purification
Gene ID:
4609
Clone Number:
ARC1534
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of HeLa cells, using Phospho-c-Myc-T58 Rabbit mAb (CABP0990) at 1:1000 dilution.Hela cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
