The Phospho-c-Myc (S62) Rabbit Monoclonal Antibody (CABP0989) is a valuable tool for researchers studying the c-Myc protein, a key regulator of cell growth and proliferation. This antibody specifically recognizes the phosphorylated form of c-Myc at serine 62, a critical post-translational modification that affects its stability and activity. Raised in rabbits, this antibody has high sensitivity and specificity for human samples, making it ideal for use in Western blot and immunohistochemistry applications. By targeting the phosphorylated form of c-Myc, researchers can gain insights into the signaling pathways and functions regulated by this protein in various biological processes, including cancer development and progression.
The Phospho-c-Myc (S62) Rabbit Monoclonal Antibody (CABP0989) is a powerful tool for investigating the role of c-Myc phosphorylation in cellular processes and disease states. Its high reactivity and specificity make it a valuable asset for studies in cancer biology, cell signaling, and drug development targeting c-Myc activity.
Product Name:
Phospho-c-Myc-S62 Rabbit Monoclonal Antibody
SKU:
CABP0989
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic phosphorylated peptide around S62 of human c-Myc (NP_002458.2).
Sequence:
PLSP S
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Synonyms:
MRTL; MYCC; c-Myc; bHLHe39; Phospho-c-Myc-S62
Positive Sample:
293T,NIH/3T3cells were treated by Forskolin (10 uM) at 37℃ for 30 minutes after serum-starvation overnight.
Conjugate:
Unconjugated
Cellular Localization:
Nucleus, nucleolus, nucleoplasm
Calculated MW:
51kDa
Observed MW:
62kDa
This gene is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini.
Purification Method:
Affinity purification
Gene ID:
4609
Clone Number:
ARC1533
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Phospho-c-Myc-S62 Rabbit mAb (CABP0989) at 1:1000 dilution.293T cells were treated by Forskolin (10 uM) at 37℃ for 30 minutes after serum-starvation overnight.NIH/3T3 cells were treated by Forskolin (30 uM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
