The Phospho-c-Jun (T91) Rabbit Monoclonal Antibody (CABP1003) is specifically designed for research involving the phosphorylation of c-Jun at threonine 91, a key regulator of gene expression and cell proliferation. This antibody, raised in rabbits, is highly reactive with human samples and has been validated for use in Western blot applications.The phosphorylation of c-Jun at threonine 91 plays a crucial role in various cellular processes, including cell growth, differentiation, and apoptosis. By targeting this specific phosphorylation site, researchers can gain insights into the signaling pathways and mechanisms involved in these processes.
This antibody allows for the detection and analysis of phospho-c-Jun (T91) in different cell types, making it an ideal tool for studies in molecular biology, signal transduction, and cancer research.Understanding the role of phospho-c-Jun (T91) is essential for unraveling its involvement in disease processes, such as cancer, where dysregulation of gene expression and cell proliferation can lead to tumor growth and progression. By studying the phosphorylation status of c-Jun at threonine 91, researchers can identify potential therapeutic targets and develop treatments that target this specific pathway.
Product Name:
Phospho-c-Jun-T91 Rabbit Monoclonal Antibody
SKU:
CABP1003
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around T91 of human c-Jun (P05412).
Sequence:
TTTP T
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB,1:500 - 1:2000 IHC-P,1:50 - 1:200
Synonyms:
AP1; p39; AP-1; cJUN; c-Jun; Phospho-c-Jun-T91
Positive Sample:
NIH/3T3+UV,NIH/3T3+Anisomycin,293T+Anisomycin
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
36kDa
Observed MW:
43kDa
This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.
Purification Method:
Affinity purification
Gene ID:
3725
Clone Number:
ARC1548
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-c-Jun-T91 Rabbit mAb (CABP1003) at 1:1000 dilution.NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes or treated by Anisomycin (25 μg/mL) at 37℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
