The Phospho-c-Jun (S63) Rabbit Monoclonal Antibody (CABP0105) is a valuable tool for researchers studying the phosphorylation of c-Jun, a component of the transcription factor AP-1 which plays a crucial role in cell proliferation, differentiation, and apoptosis. This antibody, produced in rabbits, is highly specific for detecting phosphorylated c-Jun at serine 63 in human samples, making it a reliable choice for Western blotting applications.Phosphorylation of c-Jun at serine 63 is known to regulate its activity and function in various cellular processes, including response to stress stimuli and activation of gene expression.
By targeting this specific phosphorylation site, researchers can gain insight into the signaling pathways and molecular mechanisms involved in cell signaling and gene regulation.This antibody is a valuable tool in immunology and cancer research, where dysregulation of c-Jun phosphorylation is implicated in various diseases, making it a promising target for therapeutic interventions. With its high reactivity and specificity, the Phospho-c-Jun (S63) Rabbit Monoclonal Antibody (CABP0105) is an essential component for studies aiming to unravel the complex roles of c-Jun in cellular physiology and disease pathology.
Product Name:
Phospho-c-Jun-S63 Rabbit Monoclonal Antibody
SKU:
CABP0105
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S63 of human c-Jun (P05412).
Sequence:
LTSP DV
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB,1:500 - 1:2000 IF/ICC,1:50 - 1:200
Synonyms:
AP1; p39; AP-1; cJUN; c-Jun; Phospho-c-Jun-S63
Positive Sample:
NIH/3T3+Anisomycin
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
36kDa
Observed MW:
48kDa
This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.
Purification Method:
Affinity purification
Gene ID:
3725
Clone Number:
ARC0051
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-c-Jun-S63 Rabbit mAb (CABP0105) at 1:1000 dilution.NIH/3T3 cells were treated by Anisomycin (25 μg/mL) at 37℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
