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Phospho-BRD4-T249 Polyclonal Antibody (CABP1515)

SKU:
CABP1515
$155
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Description

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Product Name:Phospho-BRD4-T249 Polyclonal Antibody
SKU:CABP1515
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic phosphorylated peptide around T249 of human BRD4 (NP_490597.1).
Tested Applications:WB
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:CAP; MCAP; HUNK1; HUNKI; FSHRG4; Phospho-BRD4-T249
Positive Sample:NIH/3T3+CA
Conjugate:Unconjugated
Cellular Localization:Chromosome, Nucleus.
Calculated MW:152kDa
Observed MW:200kDa

The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described.

Purification Method:Affinity purification
Gene ID:23476
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from NIH/3T3 cells using Phospho-BRD4-T249 Rabbit pAb (CABP1515) at 1:1000 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

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