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Phospho-BRD4-S1083 Monoclonal Antibody (CABP1235)

SKU:
CABP1235
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
€139 - €469
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-BRD4-S1083 Monoclonal Antibody
SKU:CABP1235
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:A synthetic phosphorylated peptide around S1083 of human BRD4 (NP_490597.1).
Sequence:HQSP P
Tested Applications:WB ELISA
Recommended Dilution:WB,1:100 - 1:500
Synonyms:CAP; MCAP; HUNK1; HUNKI; FSHRG4; Phospho-BRD4-S1083
Positive Sample:HeLa
Conjugate:Unconjugated
Cellular Localization:condensed nuclear chromosome, nucleoplasm, nucleus
Calculated MW:152kDa
Observed MW:200kDa

The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described.

Purification Method:Affinity purification
Gene ID:23476
Clone Number:ARC5019-01
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using Phospho-BRD4-S1083 Rabbit mAb (CABP1235) at1:500 dilution.HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.HeLa cells were treated by nocodazole (50 ng/ml) at 37℃ for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 300s.

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