The Phospho-BRCA1 (S988) Polyclonal Antibody (CABP0318) is a valuable tool for researchers studying BRCA1, a crucial tumor suppressor gene involved in DNA repair and maintenance. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications.BRCA1, a key player in maintaining genomic stability, is known to be phosphorylated at serine 988, a modification that regulates its function in DNA repair processes. The Phospho-BRCA1 (S988) antibody enables the detection and analysis of this phosphorylated form of BRCA1 in a variety of cell types, providing insights into its role in DNA damage response mechanisms.
Research into BRCA1 and its phosphorylation status is important for understanding its involvement in cancer development and progression, particularly in breast and ovarian cancers where BRCA1 mutations are prevalent. By studying the phosphorylation of BRCA1 at serine 988, researchers can uncover potential therapeutic targets and pathways for the treatment and management of these cancer types.
Product Name:
Phospho-BRCA1-S988 Rabbit Polyclonal Antibody
SKU:
CABP0318
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around S988 of human BRCA1 (NP_009225.1).
This gene encodes a 190 kD nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The BRCA1 gene contains 22 exons spanning about 110 kb of DNA. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified.
Purification Method:
Affinity purification
Gene ID:
672
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Immunohistochemistry analysis of Phospho-BRCA1-S988 in paraffin-embedded human breast cancer tissue using Phospho-BRCA1-S988 Rabbit pAb (CABP0318) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.
