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Phospho-B-Raf-S728 Rabbit Polyclonal Antibody (CABP0576)

SKU:
CABP0576
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
$503
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Description

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Product Name:Phospho-B-Raf-S728 Rabbit Polyclonal Antibody
SKU:CABP0576
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:A synthetic phosphorylated peptide around S728 of human BRAF (NP_004324.2).
Sequence:SASE P
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Synonyms:NS7; B-raf; BRAF1; RAFB1; B-RAF1; BRAF-1; Phospho-B-Raf-S728
Positive Sample:HeLa,HeLa+EGF,293,293+Insulin
Conjugate:Unconjugated
Cellular Localization:Cell membrane, Cytoplasm, Nucleus.
Calculated MW:84kDa
Observed MW:86kDa

This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome.

Purification Method:Affinity purification
Gene ID:673
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of various cell lines, using Phospho-B-Raf-S728 antibody (CABP0576).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.

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