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Phospho-B-Raf-S728 Rabbit Polyclonal Antibody (CABP0576)

SKU:
CABP0576
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
€419
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Description

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Phospho-B-Raf-S728 Rabbit Polyclonal Antibody (CABP0576)

The BRAF (Phospho S728) Polyclonal Antibody (CABP0576) is a valuable tool for researchers studying the phosphorylation of BRAF, a key signaling protein involved in cell growth and proliferation. This antibody, generated in rabbits, is highly specific for detecting phosphorylated BRAF at serine 728 in human samples, making it ideal for Western blot applications.Phosphorylation of BRAF at serine 728 is known to regulate the activity of BRAF and its downstream signaling pathways, impacting cellular processes such as cell cycle progression and apoptosis.

By detecting and monitoring the phosphorylation status of BRAF, researchers can gain insight into the mechanisms underlying cell signaling and potential therapeutic targets for diseases like cancer.With its high reactivity and specificity, the BRAF (Phospho S728) Polyclonal Antibody is a valuable asset for studies in cancer research and drug development, providing researchers with a reliable tool for investigating the role of BRAF phosphorylation in disease progression and treatment response.

Product Name:Phospho-B-Raf-S728 Rabbit Polyclonal Antibody
SKU:CABP0576
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:A synthetic phosphorylated peptide around S728 of human BRAF (NP_004324.2).
Sequence:SASE P
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Synonyms:NS7; B-raf; BRAF1; RAFB1; B-RAF1; BRAF-1; Phospho-B-Raf-S728
Positive Sample:HeLa,HeLa+EGF,293,293+Insulin
Conjugate:Unconjugated
Cellular Localization:Cell membrane, Cytoplasm, Nucleus.
Calculated MW:84kDa
Observed MW:86kDa

This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome.

Purification Method:Affinity purification
Gene ID:673
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of various cell lines, using Phospho-B-Raf-S728 antibody (CABP0576).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.

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