The Phospho-BRAF (S445) Polyclonal Antibody (CABP0012) is a valuable tool for researchers studying BRAF, a key player in the MAPK signaling pathway commonly mutated in various cancers. This antibody, generated in rabbits, exhibits high specificity and sensitivity in detecting phosphorylation of BRAF at Serine 445 in human samples, making it suitable for Western blot analysis.BRAF is a critical protein kinase involved in cell growth, proliferation, and differentiation, with aberrant activation contributing to tumorigenesis. Phosphorylation at Serine 445 has been implicated in regulating BRAF activity and downstream signaling pathways, making it a crucial post-translational modification to study in cancer biology.
This antibody enables precise detection and quantification of phosphorylated BRAF (S445) in different cell types, advancing research in oncology and drug development.Understanding the phosphorylation status of BRAF at specific sites, such as Serine 445, is essential for unraveling the complex mechanisms driving cancer progression and resistance to targeted therapies. By utilizing the Phospho-BRAF (S445) Polyclonal Antibody, researchers can gain insights into the functional role of BRAF phosphorylation in different cancer types, paving the way for novel therapeutic strategies targeting this critical signaling pathway.
Product Name:
Phospho-B-Raf-S445 Rabbit Polyclonal Antibody
SKU:
CABP0012
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
A synthetic phosphorylated peptide around S445 of human BRAF (NP_004324.2).
This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome.
Purification Method:
Affinity purification
Gene ID:
673
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of Raji cells, using Phospho-B-Raf-S445 pAb (CABP0012) at 1:2000 dilution or BRAF antibody (CAB0038).Raji cells were treated by PMA/TPA (200nM) for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
