Phospho-BLNK (Tyr96) Antibody (PACO24050)
- SKU:
- PACO24050
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- WB
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
Phospho-BLNK (Tyr96) Antibody (PACO24050)
The Phospho-BLNK (Tyr96) Antibody (PAC024050) is a valuable tool for researchers studying signaling pathways involved in immune responses. This antibody, specifically designed to detect phosphorylation at Tyr96 of the BLNK protein, is highly specific and sensitive, making it ideal for use in Western blot and immunohistochemistry applications.BLNK, also known as B Cell Linker Protein, plays a critical role in B cell receptor signaling and activation. Phosphorylation at Tyr96 is known to regulate the function of BLNK, influencing downstream signaling cascades that are essential for proper immune cell activation and response.
Research into the phosphorylation status of BLNK at Tyr96 provides valuable insights into the regulation of immune cell function and can lead to a better understanding of diseases such as autoimmune disorders and cancer.The Phospho-BLNK (Tyr96) Antibody (PAC024050) is produced in rabbits and validated for use with human samples, ensuring reliable and consistent results in experiments. By enabling the detection and analysis of phosphorylated BLNK, this antibody is a powerful tool for researchers seeking to unravel the complexities of immune signaling pathways and develop targeted therapies for immune-related diseases.
Antibody Name: | Phospho-BLNK (Tyr96) Antibody (PACO24050) |
Antibody SKU: | PACO24050 |
Size: | 100ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:1000 |
Species Reactivity: | Human, Mouse |
Immunogen: | Peptide sequence around phosphorylation site of tyrosine 96(D-S-Y(p)-E-P) derived from Human BLNK . |
Form: | Liquid |
Storage Buffer: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western blot analysis of extracts from COLO205 tissue using BLNK (Phospho-Tyr96) antibody.The lane on the right is treated with the antigen-specific peptide. |
Background: | Functions as a central linker protein, downstream of the B-cell receptor (BCR), bridging the SYK kinase to a multitude of signaling pathways and regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation. Important for the activation of NF-kappa-B and NFAT. Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca2+ mobilization and is required for trafficking of the BCR to late endosomes. However, does not seem to be required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. |
Synonyms: | B-cell linker protein; LY57; SLP-65; SLP65; |
UniProt Protein Function: | BLNK: an adaptor protein that bridges the B-cell receptor-associated kinases (BCR) with a multitude of signaling pathways, regulating biologic outcomes of B-cell function and development. Plays an important role in BCR-mediated PLCG1 activation and Ca(2 ) mobilization. Does not seem to be not required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition. Following BCR activation, phosphorylated on tyrosine residues by SYK and LYN. When phosphorylated, serves as a scaffold to assemble downstream targets of antigen activation, including PLCG1, VAV1, GRB2 and NCK1. Its phosphorylation is required for both Ca(2 ) and MAPK signaling pathways. Defects in BLNK are the cause of hypoglobulinemia and absent B-cells.It has tumor supressor activity that is lost in many cases of childhood pre-B acute lymphoblastic leukemia (ALL). Two alternatively spliced isoforms have been described; these are differentially involved in activation and apoptosis of B lymphocytes. |
UniProt Protein Details: | Protein type:Adaptor/scaffold Chromosomal Location of Human Ortholog: 10q23.2-q23.33 Cellular Component: cytoplasm; cytosol; plasma membrane Molecular Function:protein binding; SH3/SH2 adaptor activity; transmembrane receptor protein tyrosine kinase adaptor protein activity Biological Process: humoral immune response; inflammatory response Disease: Agammaglobulinemia 4, Autosomal Recessive |
NCBI Summary: | This gene encodes a cytoplasmic linker or adaptor protein that plays a critical role in B cell development. This protein bridges B cell receptor-associated kinase activation with downstream signaling pathways, thereby affecting various biological functions. The phosphorylation of five tyrosine residues is necessary for this protein to nucleate distinct signaling effectors following B cell receptor activation. Mutations in this gene cause hypoglobulinemia and absent B cells, a disease in which the pro- to pre-B-cell transition is developmentally blocked. Deficiency in this protein has also been shown in some cases of pre-B acute lymphoblastic leukemia. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, May 2012] |
UniProt Code: | Q8WV28 |
NCBI GenInfo Identifier: | 82592659 |
NCBI Gene ID: | 29760 |
NCBI Accession: | Q8WV28.2 |
UniProt Secondary Accession: | Q8WV28,O75498, O75499, Q2MD49, |
UniProt Related Accession: | Q8WV28 |
Molecular Weight: | 44,451 Da |
NCBI Full Name: | B-cell linker protein |
NCBI Synonym Full Names: | B-cell linker |
NCBI Official Symbol: | BLNKÂ Â |
NCBI Official Synonym Symbols: | bca; AGM4; BASH; LY57; SLP65; BLNK-S; SLP-65Â Â |
NCBI Protein Information: | B-cell linker protein |
UniProt Protein Name: | B-cell linker protein |
UniProt Synonym Protein Names: | B-cell adapter containing a SH2 domain protein; B-cell adapter containing a Src homology 2 domain protein; Cytoplasmic adapter protein; Src homology 2 domain-containing leukocyte protein of 65 kDa; SLP-65 |
Protein Family: | B-cell linker protein |
UniProt Gene Name: | BLNKÂ Â |
UniProt Entry Name: | BLNK_HUMAN |
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