The Phospho BLM (Thr99) Antibody (PAC024377) is specifically designed for researchers studying the BLM protein and its phosphorylation at threonine 99. BLM is a DNA helicase involved in maintaining genome stability and function. Phosphorylation at Thr99 plays a crucial role in regulating the activity of BLM in DNA repair processes.Raised in rabbits, this antibody exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding to the phosphorylated form of BLM, this antibody enables precise detection and analysis of BLM Thr99 phosphorylation in various cell types.
It is an essential tool for studies in molecular biology, DNA repair mechanisms, and cancer research.Understanding the phosphorylation status of BLM at Thr99 is critical for unraveling its functions in DNA repair pathways and its implications in cancer development and progression. This antibody provides researchers with a reliable tool for investigating the role of BLM phosphorylation in genetic stability and the potential for targeted therapies in cancer treatment.
Peptide sequence around phosphorylation site of threonine 99 (Q-E-T(p)-Q-R) derived from Human Bloom Syndrome.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from HepG2 cells using Bloom Syndrome Protein (Phospho-Thr99) Antibody.The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human heart tissue, using Bloom Syndrome Protein (Phospho-Thr99) antibody (left)or the same antibody preincubated with blocking peptide (right).
Background:
Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA. Negatively regulates sister chromatid exchange (SCE).
Synonyms:
RECQ2; RECQL3; type 2; EC 3.6.1; RecQ protein-like 3
UniProt Protein Function:
BLM: a magnesium-dependent ATP-dependent DNA-helicase that unwinds single- and double-stranded DNA in a 3'-5' direction. A member of the RecQ helicase family that is required for genome stability. Participates in DNA replication, recombination and repair. Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBS1 protein complex which is a dynamic process changing throughout the cell cycle and within subnuclear domains. Interacts with ubiquitinated FANCD2.Protein type: DNA repair, damage; Helicase; DNA replication; EC 3.6.4.12Chromosomal Location of Human Ortholog: 15q26.1Cellular Component: chromosome, telomeric region; cytoplasm; lateral element; nuclear chromosome; nuclear matrix; nucleolus; nucleoplasm; nucleus; PML bodyMolecular Function: ATP binding; ATP-dependent 3'-5' DNA helicase activity; ATP-dependent DNA helicase activity; ATP-dependent helicase activity; ATPase activity; bubble DNA binding; four-way junction helicase activity; G-quadruplex DNA binding; helicase activity; p53 binding; protein binding; single-stranded DNA bindingBiological Process: DNA double-strand break processing; DNA replication; DNA synthesis during DNA repair; double-strand break repair via homologous recombination; mitotic cell cycle G2/M transition DNA damage checkpoint; negative regulation of cell division; negative regulation of DNA recombination; positive regulation of transcription, DNA-dependent; protein oligomerization; protein sumoylation; regulation of cyclin-dependent protein kinase activity; replication fork processing; response to DNA damage stimulus; response to X-ray; strand displacementDisease: Bloom Syndrome
UniProt Protein Details:
NCBI Summary:
The Bloom syndrome gene product is related to the RecQ subset of DExH box-containing DNA helicases and has both DNA-stimulated ATPase and ATP-dependent DNA helicase activities. Mutations causing Bloom syndrome delete or alter helicase motifs and may disable the 3'-5' helicase activity. The normal protein may act to suppress inappropriate recombination. [provided by RefSeq, Jul 2008]
