The Phospho Bcl-2 (S87) Polyclonal Antibody (CABP1071) is a valuable tool for researchers studying the phosphorylation of Bcl-2 at serine 87, a key regulatory site involved in apoptosis and cell survival pathways. This rabbit polyclonal antibody is highly specific to human samples and has been validated for use in Western blot applications. By binding to the phosphorylated form of Bcl-2 at serine 87, this antibody allows for the detection and analysis of this important protein modification in various cell types.Bcl-2 is a critical regulator of apoptosis, and phosphorylation at serine 87 has been shown to affect its anti-apoptotic function.
Understanding the role of phosphorylated Bcl-2 in cell survival and death signaling pathways is essential for unraveling the mechanisms of cancer development and progression. This antibody provides researchers with a powerful tool to investigate the functional significance of Bcl-2 phosphorylation in cancer biology and other disease states where dysregulation of apoptosis is implicated.
Product Name:
Phospho-Bcl-2-S87 Rabbit Polyclonal Antibody
SKU:
CABP1071
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
A synthetic phosphorylated peptide around S87 of human Bcl-2 (NP_000624.2).
This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
596
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from C6 cells, using Phospho-Bcl-2-S87 Rabbit pAb (CABP1071) at 1:1000 dilution.C6 cells were treated by Paclitaxel (100 nM) at 37℃ for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
