The Phospho-BCAR1 (Y410) Polyclonal Antibody (CABP0789) is a valuable tool for researchers studying BCAR1, a key protein involved in cell signaling pathways and cancer progression. This antibody, produced using rabbits, is highly specific to human BCAR1 samples and has been validated for use in Western blot applications. It targets the phosphorylated Y410 residue of the BCAR1 protein, allowing for precise detection and analysis in various cell types.BCAR1, also known as p130Cas, is a focal adhesion protein that plays a crucial role in cell migration, invasion, and survival. Phosphorylation of the Y410 residue in BCAR1 is linked to its activation and involvement in promoting tumor growth and metastasis.
Understanding the signaling pathways mediated by phosphorylated BCAR1 is essential for unraveling the mechanisms underlying cancer progression and identifying potential targets for therapeutic intervention.Researchers studying cancer biology, cell signaling, and metastasis will find the Phospho-BCAR1 (Y410) Polyclonal Antibody to be a valuable asset in their studies. Its high specificity and sensitivity make it an ideal tool for investigating the role of BCAR1 in cancer development and progression, ultimately leading to new insights and potential therapeutic options for cancer treatment.
Product Name:
Phospho-BCAR1-Y410 Rabbit Polyclonal Antibody
SKU:
CABP0789
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic phosphorylated peptide around Y410 of human BCAR1 (NP_055382.2).
The protein encoded by this gene is a member of the Crk-associated substrate (CAS) family of scaffold proteins, characterized by the presence of multiple protein-protein interaction domains and many serine and tyrosine phosphorylation sites. The encoded protein contains a Src-homology 3 (SH3) domain, a proline-rich domain, a substrate domain which contains 15 repeat of the YxxP consensus phosphorylation motif for Src family kinases, a serine-rich domain, and a bipartite Src-binding domain, which can bind both SH2 and SH3 domains. This adaptor protein functions in multiple cellular pathways, including in cell motility, apoptosis and cell cycle control. Dysregulation of this gene can have a wide range of effects, affecting different pathways, including cardiac development, vascular smooth muscle cells, liver and kidney function, endothelial migration, and cancer.
Purification Method:
Affinity purification
Gene ID:
9564
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-BCAR1-Y410 Rabbit pAb (CABP0789) at 1:5000 dilution. HeLa cells were treated by Pervanadate (1nM) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
