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Phospho-BCAR1-Y165 Rabbit Polyclonal Antibody (CABP0813)

SKU:
CABP0813
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
$503
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-BCAR1-Y165 Rabbit Polyclonal Antibody
SKU:CABP0813
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic phosphorylated peptide around Y165 of human BCAR1 (NP_055382.2).
Sequence:DLYQ V
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Synonyms:CAS; CAS1; CASS1; CRKAS; P130Cas; Phospho-BCAR1-Y165
Positive Sample:PC-12
Conjugate:Unconjugated
Cellular Localization:Cell junction, Cytoplasm, focal adhesion.
Calculated MW:93kDa
Observed MW:130kDa

The protein encoded by this gene is a member of the Crk-associated substrate (CAS) family of scaffold proteins, characterized by the presence of multiple protein-protein interaction domains and many serine and tyrosine phosphorylation sites. The encoded protein contains a Src-homology 3 (SH3) domain, a proline-rich domain, a substrate domain which contains 15 repeat of the YxxP consensus phosphorylation motif for Src family kinases, a serine-rich domain, and a bipartite Src-binding domain, which can bind both SH2 and SH3 domains. This adaptor protein functions in multiple cellular pathways, including in cell motility, apoptosis and cell cycle control. Dysregulation of this gene can have a wide range of effects, affecting different pathways, including cardiac development, vascular smooth muscle cells, liver and kidney function, endothelial migration, and cancer.

Purification Method:Affinity purification
Gene ID:9564
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from PC-12 cells, using Phospho-BCAR1-Y165 Rabbit pAb (CABP0813) at 1:1000 dilution. PC-12 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

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