The Phospho-Aurka (T288), Aurkb (T232), Aurkc (T198) Polyclonal Antibody (CABP0948) is a valuable tool for researchers studying the phosphorylation of the Aurora kinases, key regulators of cell division and mitosis. This antibody, generated in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to the phosphorylated forms of Aurka, Aurkb, and Aurkc, this antibody allows for the detection and analysis of these proteins in a variety of cell types.The Aurora kinases play a crucial role in the regulation of cell cycle progression, ensuring accurate chromosome segregation and cell division. Phosphorylation of these kinases at specific sites, such as T288 in Aurka, T232 in Aurkb, and T198 in Aurkc, is known to impact their activity and function.
By targeting these phosphorylation sites, the CABP0948 antibody enables researchers to explore the role of these kinases in processes such as cell proliferation, apoptosis, and tumorigenesis.Studies have shown that dysregulation of the Aurora kinases is associated with various cancers and other diseases, making them attractive targets for therapeutic intervention. By using the Phospho-Aurka (T288), Aurkb (T232), Aurkc (T198) Polyclonal Antibody (CABP0948) in their research, scientists can gain valuable insights into
The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Purification Method:
Affinity purification
Gene ID:
6790/9212/6795
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-AURKA-T288/AURKB-T232/AURKC-T198 Rabbit pAb (CABP0948) at 1:1000 dilution.Hela cells were treated by ATP(5 mM) at 30℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
