The Phospho-ATR (S428) Polyclonal Antibody (CABP0676) is a valuable tool for researchers studying the ATR protein, a key regulator of DNA damage response pathways. This antibody, produced in rabbits, is highly specific for detecting phosphorylated ATR at serine 428 in human samples. It has been validated for use in Western blot applications, enabling researchers to assess ATR activation in various cell types.The phosphorylation of ATR at serine 428 is known to play a crucial role in the activation of the ATR signaling pathway in response to DNA damage. By targeting this specific phosphorylation site, researchers can gain insights into the mechanisms underlying DNA damage repair and cell cycle checkpoint control.
This antibody is an important resource for studies investigating the role of ATR in maintaining genomic stability and preventing the accumulation of mutations that can lead to cancer and other genetic diseases.Overall, the Phospho-ATR (S428) Polyclonal Antibody (CABP0676) offers researchers a powerful tool for exploring the function of ATR in DNA damage response pathways, making it a valuable asset in the fields of molecular biology, cancer research, and drug development.
Product Name:
Phospho-ATR-S428 Rabbit Polyclonal Antibody
SKU:
CABP0676
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S428 of human ATR (NP_001175.2).
The protein encoded by this gene is a serine/threonine kinase and DNA damage sensor, activating cell cycle checkpoint signaling upon DNA stress. The encoded protein can phosphorylate and activate several proteins involved in the inhibition of DNA replication and mitosis, and can promote DNA repair, recombination, and apoptosis. This protein is also important for fragile site stability and centrosome duplication. Defects in this gene are a cause of Seckel syndrome 1.
Purification Method:
Affinity purification
Gene ID:
545
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from 293T cells, using Phospho-ATR-S428 Rabbit pAb (CABP0676) at 1:400 dilution.293T cells were treated by UV at room temperature for 15-30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
