The Phospho-ATR (S428) Monoclonal Antibody is a highly specific tool for researchers studying the ATR protein and its phosphorylation at serine 428. ATR is a key regulator of the DNA damage response pathway, playing a critical role in maintaining genomic stability and preventing the accumulation of mutations that can lead to cancer. This monoclonal antibody, produced using cutting-edge technology, offers high sensitivity and specificity in detecting phosphorylated ATR at serine 428 in human samples. It is validated for use in Western blot applications, allowing for precise and reliable quantification of phospho-ATR levels in various cell types and experimental conditions.
By targeting the phosphorylated form of ATR, researchers can gain valuable insights into the activity of this important protein in cellular responses to DNA damage and replication stress. This antibody is an essential tool for advancing our understanding of DNA damage signaling pathways and for identifying potential therapeutic targets for cancer treatment.
Product Name:
Phospho-ATR-S428 Monoclonal Antibody
SKU:
CABP1358
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around S428 of human ATR (NP_001175.2).
Sequence:
GISP K
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:1000 - 1:5000
Synonyms:
FRP1; MEC1; SCKL; FCTCS; SCKL1; Phospho-ATR-S428
Positive Sample:
293T+UV
Conjugate:
Unconjugated
Cellular Localization:
Chromosome, Nucleus, PML body.
Calculated MW:
301kDa
Observed MW:
300kDa
The protein encoded by this gene is a serine/threonine kinase and DNA damage sensor, activating cell cycle checkpoint signaling upon DNA stress. The encoded protein can phosphorylate and activate several proteins involved in the inhibition of DNA replication and mitosis, and can promote DNA repair, recombination, and apoptosis. This protein is also important for fragile site stability and centrosome duplication. Defects in this gene are a cause of Seckel syndrome 1.
Purification Method:
Affinity purification
Gene ID:
545
Clone Number:
ARC55118
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using Phospho-ATR-S428 antibody (CABP1358) at1:2000 dilution.293T cells were treated by UV at room temperature for 15-30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
