The Phospho-ATM (S1981) Polyclonal Antibody (CABP0008) is a crucial tool for researchers studying the ATM protein, a key player in DNA damage response pathways. This antibody, generated in rabbits, exhibits high specificity and sensitivity for detecting levels of phosphorylated ATM at serine 1981 in human samples. Validated for use in Western blot applications, it enables precise quantification and visualization of ATM activation in various cell types.ATM, a member of the PI3/PI4-kinase family, is known to phosphorylate numerous downstream targets involved in cell cycle regulation, DNA repair, and apoptosis. Phosphorylation of ATM at serine 1981 is a hallmark of its activation in response to double-strand DNA breaks, playing a crucial role in initiating DNA damage repair mechanisms.
Understanding the dynamics of ATM activation is essential for unraveling its intricate functions in maintaining genomic integrity and preventing the accumulation of mutations that can lead to cancer and other genetic diseases. By providing a reliable means to assess ATM phosphorylation status, the Phospho-ATM (S1981) Polyclonal Antibody (CABP0008) offers researchers a valuable tool for investigating the molecular mechanisms underlying DNA damage response pathways. Its application extends to fields such as cancer biology, radiation therapy, and drug development, where targeting ATM signaling holds promise for improving treatment outcomes and overcoming resistance to genotoxic therapies.
Product Name:
Phospho-ATM-S1981 Rabbit Polyclonal Antibody
SKU:
CABP0008
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic phosphorylated peptide around S1981 of human ATM (NP_000042.3).
The protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder.
Purification Method:
Affinity purification
Gene ID:
472
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using (CABP0008) at 1:1000 dilution.293T cells were treated by Doxorubicin (0.5 uM) at 37℃ for 24 hours.293T cells were treated by UV at room temperature for 15-30 minutes.NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
