The Phospho-ATF4 (S245) Polyclonal Antibody (CABP0309) is a valuable tool for researchers studying ATF4, a key transcription factor involved in the cellular stress response. This antibody, generated in rabbits, exhibits high reactivity with human samples and is suitable for Western blot applications. By targeting the phosphorylated form of ATF4 at serine 245, this antibody allows for the detection and analysis of ATF4 activation in various cell types, making it ideal for investigations in the fields of molecular biology, cell signaling, and cancer research.
Phosphorylation of ATF4 at serine 245 plays a critical role in regulating its transcriptional activity and impact on cellular processes such as apoptosis, autophagy, and metabolism. Understanding the phosphorylation status of ATF4 is essential for deciphering its role in stress responses and disease pathogenesis, particularly in conditions such as cancer, neurodegenerative disorders, and metabolic diseases. This antibody serves as a valuable tool for exploring the function of ATF4 and its potential as a therapeutic target in these contexts.
Product Name:
Phospho-ATF4-S245 Rabbit Polyclonal Antibody
SKU:
CABP0309
Size:
100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A phospho specific peptide corresponding to residues surrounding S245 of human ATF4
This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromosome at q28 in a region containing a large inverted duplication.
Purification Method:
Affinity purification
Gene ID:
468
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells using Phospho-ATF4-S245 Rabbit pAb (CABP0309).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.
