The Phospho-ATF2 (T69/71) Rabbit Polyclonal Antibody (CABP0913) is a valuable tool for researchers studying ATF2 phosphorylation, a crucial event in cell signaling and gene expression regulation. ATF2 is a transcription factor that plays a key role in response to stress and growth factors, making it a target of interest in cancer research and other fields.This antibody, generated in rabbits, is highly specific for the phosphorylation of Threonine 69 and 71 on ATF2, allowing for precise detection and analysis of this important post-translational modification.
It has been validated for use in Western blot applications with human samples, providing reliable and accurate results.By using the Phospho-ATF2 (T69/71) Rabbit Polyclonal Antibody, researchers can gain insights into the regulation of ATF2 activity in various cellular processes, potentially leading to advancements in understanding diseases such as cancer and neurodegenerative disorders. This antibody is a valuable tool for those studying signal transduction pathways and gene expression regulation.
Product Name:
Phospho-ATF2-T69/71 Rabbit Polyclonal Antibody
SKU:
CABP0913
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around T69 & T71 of human ATF2 (NP_001871.2?).
This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. This protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. The encoded protein may also be involved in cell's DNA damage response independent of its role in transcriptional regulation. Several alternatively spliced transcript variants have been found for this gene
Purification Method:
Affinity purification
Gene ID:
1386
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-ATF2-T69/71 Rabbit pAb (CAB2155).293T cells were treated by UV at room temperature for 15-30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
