null

Phospho-ARHGAP35 (Tyr1105) Antibody (PACO23932)

SKU:
PACO23932
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Applications:
ELISA
WB
Antibody Type:
Polyclonal Antibody
Conjugation:
Unconjugated
€549
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS

Phospho-ARHGAP35 (Tyr1105) Antibody (PACO23932)

The Phospho-ARHGAP35 (Tyr1105) Polyclonal Antibody (PAC023932) is a valuable tool for researchers studying ARHGAP35, a protein involved in cell signaling pathways and cytoskeletal organization. Phospho-ARHGAP35 plays a critical role in regulating cellular processes such as cell adhesion, migration, and proliferation. This antibody, generated in rabbits, has been shown to specifically recognize the phosphorylated form of ARHGAP35 at Tyr1105 in human samples. It is validated for use in Western blot applications, allowing for the detection and analysis of phospho-ARHGAP35 protein in various cell types.

Research on ARHGAP35 and its phosphorylation is important for understanding its role in cancer progression, cell motility, and other cellular functions. By targeting the phosphorylated form of ARHGAP35, researchers can gain insights into the signaling pathways and molecular mechanisms that drive these processes, providing opportunities for the development of targeted therapies in cancer and other diseases.

Antibody Name:Phospho-ARHGAP35 (Tyr1105) Antibody (PACO23932)
Antibody SKU:PACO23932
Size:100ul
Host Species:Rabbit
Tested Applications:ELISA, WB
Recommended Dilutions:ELISA:1:2000-1:10000, WB:1:500-1:1000
Species Reactivity:Human, Mouse, Rat
Immunogen:Peptide sequence around phosphorylation site of Tyrosine 1105(N-I-Y(p)-S-V) derived from Human GRF-1.
Form:Liquid
Storage Buffer:Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:Polyclonal
Isotype:IgG
Conjugate:Non-conjugated
Product ImageWestern blot analysis of extracts from Mouse brain tissue using GRF-1 (Phospho-Tyr1105) Antibody.The lane on the left is treated with the antigen-specific peptide.
Background:Represses transcription of the glucocorticoid receptor by binding to the cis-acting regulatory sequence 5'-GAGAAAAGAAACTGGAGAAACTC-3'. May participate in the regulation of retinal development and degeneration. May transduce signals from p21-ras to the nucleus, acting via the ras GTPase-activating protein (GAP). May also act as a tumor suppressor.
Synonyms:GRLF1, P190A, P190-A, p190RhoGAP, p190ARhoGAP
UniProt Protein Function:GRF-1: the human glucocorticoid receptor DNA binding factor, which associates with the promoter region of the glucocorticoid receptor gene (hGR gene), is a repressor of glucocorticoid receptor transcription. May participate in the regulation of retinal development and degeneration. May transduce signals from p21-ras to the nucleus, acting via the ras GTP-ase activating protein (GAP). May also act as a tumor suppressor. Two splice variant isoforms have been described. Protein type: Motility/polarity/chemotaxis; GAPs, Rac/Rho; Transcription factor; GAPs; Tumor suppressorChromosomal Location of Human Ortholog: 19q13.3Cellular Component: cytosol; nucleus; actin cytoskeletonMolecular Function: GTP binding; DNA binding; transcription corepressor activityBiological Process: regulation of cell shape; axon guidance; regulation of small GTPase mediated signal transduction; camera-type eye development; negative regulation of Rho protein signal transduction; transcription, DNA-dependent; small GTPase mediated signal transduction; neural tube closure; forebrain development; negative regulation of vascular permeability; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent
UniProt Protein Details:
NCBI Summary:The human glucocorticoid receptor DNA binding factor, which associates with the promoter region of the glucocorticoid receptor gene (hGR gene), is a repressor of glucocorticoid receptor transcription. The amino acid sequence deduced from the cDNA sequences show the presence of three sequence motifs characteristic of a zinc finger and one motif suggestive of a leucine zipper in which 1 cysteine is found instead of all leucines. The GRLF1 enhances the homologous down-regulation of wild-type hGR gene expression. Biochemical analysis suggests that GRLF1 interaction is sequence specific and that transcriptional efficacy of GRLF1 is regulated through its interaction with specific sequence motif. The level of expression is regulated by glucocorticoids. [provided by RefSeq, Jul 2008]
UniProt Code:Q9NRY4
NCBI GenInfo Identifier:150417981
NCBI Gene ID:2909
NCBI Accession:NP_004482.4
UniProt Secondary Accession:Q9NRY4,Q14452, Q9C0E1, A7E2A4
UniProt Related Accession:Q9NRY4
Molecular Weight:155kDa
NCBI Full Name:rho GTPase-activating protein 35
NCBI Synonym Full Names:Rho GTPase activating protein 35
NCBI Official Symbol:ARHGAP35  
NCBI Official Synonym Symbols:GRF-1; GRLF1; P190A; P190-A; p190RhoGAP; p190ARhoGAP  
NCBI Protein Information:rho GTPase-activating protein 35; rho GAP p190A; glucocorticoid receptor repression factor 1; glucocorticoid receptor DNA binding factor 1; glucocorticoid receptor DNA-binding factor 1
UniProt Protein Name:Rho GTPase-activating protein 35
UniProt Synonym Protein Names:Glucocorticoid receptor DNA-binding factor 1; Glucocorticoid receptor repression factor 1; GRF-1; Rho GAP p190A
Protein Family:
UniProt Gene Name:ARHGAP35  
UniProt Entry Name:RHG35_HUMAN
Antibodies
Phospho-ARHGAP35 (Y1087) Antibody (PACO02800)
Phospho-ARHGAP35 (Y1105) Antibody (PACO02801)
ARHGAP35 Antibody (PACO03988)
Secondary Antibody
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)
Recommended Products
Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011)
Anti-HRP-conjugated Beta Actin Antibody (CABC028)

Related Products