The Phospho-AMPKalpha1 (S496) Rabbit Monoclonal Antibody (CABP1002) is a cutting-edge tool designed for studying the phosphorylation status of AMPKalpha1, a key regulator of cellular energy metabolism and nutrient sensing pathways. This antibody, generated in rabbits, exhibits high sensitivity and specificity towards human samples and is validated for use in Western blot applications. AMP-activated protein kinase (AMPK) is a central player in cellular energy homeostasis, regulating processes such as glucose uptake and fatty acid oxidation. The phosphorylation of AMPK at serine 496 (S496) is known to modulate its activity, impacting downstream signaling cascades involved in metabolism, cell growth, and autophagy.
By specifically detecting phosphorylated AMPKalpha1 at S496, this antibody enables researchers to investigate the impact of post-translational modifications on AMPK function in various cellular contexts. With implications in metabolic disorders, cancer, and cardiovascular diseases, understanding the regulation of AMPK phosphorylation can offer valuable insights for developing targeted therapeutics and interventions. Researchers in the fields of metabolism, cell biology, and cancer research will find the Phospho-AMPKalpha1 (S496) Rabbit Monoclonal Antibody (CABP1002) to be a valuable asset for unraveling the complex mechanisms governing cellular energy balance and metabolic regulation.
Product Name:
Phospho-AMPKα1-S496 Rabbit Monoclonal Antibody
SKU:
CABP1002
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse
Immunogen:
A synthetic phosphorylated peptide around S496 of human AMPKα1 (Q13131).
Sequence:
SGSV S
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
AMPK; AMPKa1; AMPK alpha 1; Phospho-AMPKα1-S496
Positive Sample:
C2C12+oligomycin
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
64kDa
Observed MW:
64kDa
The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Purification Method:
Affinity purification
Gene ID:
5562
Clone Number:
ARC1547
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of C2C12 cells, using Phospho-AMPKα1-S496 Rabbit mAb (CABP1002) at 1:1000 dilution.C2C12 cells were treated by oligomycin (0.5 μM) at 37℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.
