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Phospho-AMPKa1-T183 + AMPKa2-T172 Monoclonal Antibody (CABP1345)

SKU:
CABP1345
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Applications:
WB
$167 - $731
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-AMPKa1-T183 + AMPKa2-T172 Monoclonal Antibody
SKU:CABP1345
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Mouse
Immunogen:A synthetic phosphorylated peptide around T183 of human AMPKa1 (NP_006242.5).
Sequence:LRTS C
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:AMPKa1/AMPKa2; Phospho-AMPKa1-T183 + AMPKa2-T172
Positive Sample:C2C12+oligomycin
Cellular Localization:Apical plasma membrane, Cytoplasm, Cytosol, Nuclear speck, Nucleoplasm, nucleus
Calculated MW:64kDa/65kDa/62kDa
Observed MW:62kDa

The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Purification Method:Affinity purification
Gene ID:5562/5563
Clone Number:ARC53386
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from C2C12 cells, using Phospho-AMPKa1-T183 + AMPKa2-T172 Rabbit mAb (CABP1345) at1:1000 dilution.C2C12 cells were treated by oligomycin (0.5 uM) at 37℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.

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