The Phospho-AMPKα1 (T183)/AMPKα2 (T172) Monoclonal Antibody is a valuable tool for researchers studying AMP-activated protein kinase (AMPK) signaling pathways. This antibody, derived from mouse monoclonal cells, specifically targets the phosphorylated forms of AMPKα1 at threonine 183 and AMPKα2 at threonine 172. It is highly reactive with human, mouse, and rat samples and has been validated for use in Western blot applications.AMPK is a key regulator of cellular energy balance and metabolism, making it a crucial player in processes such as glucose uptake, fatty acid oxidation, and autophagy. Phosphorylation at specific sites, such as T183 on AMPKα1 and T172 on AMPKα2, is known to activate AMPK and modulate its downstream signaling cascades.
By using this antibody to detect and analyze phospho-AMPKα1/T183 and phospho-AMPKα2/T172, researchers can gain insights into the role of AMPK in various biological processes.The Phospho-AMPKα1 (T183)/AMPKα2 (T172) Monoclonal Antibody is an essential tool for studies in cellular metabolism, energy homeostasis, and metabolic disorders. Its specificity and reliability make it ideal for investigations into the therapeutic potential of targeting AMPK signaling in diseases like diabetes, obesity, and cancer
The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Purification Method:
Affinity purification
Gene ID:
5562/5563
Clone Number:
ARC53386
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from C2C12 cells, using Phospho-AMPKa1-T183 + AMPKa2-T172 Rabbit mAb (CABP1345) at1:1000 dilution.C2C12 cells were treated by oligomycin (0.5 uM) at 37℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
