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Phospho-AMPKα1-T183+AMPKα2-T172 Monoclonal Antibody (CABP1441)

SKU:
CABP1441
$167
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Description

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Product Name:Phospho-AMPKα1-T183+AMPKα2-T172 Monoclonal Antibody
SKU:CABP1441
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic phosphorylated peptide around T183/T172 of human PRKAA1/PRKAA2 (NP_006242.5/NP_006243.2).
Sequence:LRTS C
Tested Applications:WB ELISA
Recommended Dilution:WB,1:1000 - 1:5000
Synonyms:AMPKa1/AMPKa2; Phospho-AMPKα1-T183+AMPKα2-T172
Positive Sample:C2C12+HGF(m),C6+AICAR
Cellular Localization:Apical plasma membrane, Cytoplasm, Cytosol, Nuclear speck, Nucleoplasm, Nucleus
Calculated MW:64kDa/65kDa/62kDa
Observed MW:62kDa

The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Purification Method:Affinity purification
Gene ID:5562/5563
Clone Number:ARC62278
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from C2C12 cells, using Phospho-AMPKa1-T183/AMPKa2-T172 Rabbit mAb (CABP1441) at 1:1000 dilution.C2C12 cells were treated by mHGF(50ng/uL).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

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