The Phospho-AMPK (1-T183/2-T172) Monoclonal Antibody is a highly specific tool for research involving the AMPK protein, a key regulator of cellular energy metabolism and a potential target for various diseases, including cancer and diabetes. This antibody, developed using advanced monoclonal antibody technology, is reactive with both AMPK isoforms in various species, making it ideal for widespread use in experiments such as Western blotting and immunofluorescence.The phosphorylation of AMPK at threonine residues 183 and 172 is crucial for its activation and subsequent signaling pathways that regulate cellular energy balance, making this antibody particularly valuable for studies focusing on metabolic disorders and related conditions.
By facilitating the detection and quantification of phosphorylated AMPK, researchers can gain insights into the mechanisms underlying disease pathogenesis and develop new therapeutic interventions targeting AMPK activity.Overall, the Phospho-AMPK (1-T183/2-T172) Monoclonal Antibody is a powerful tool for investigating the complex role of AMPK in health and disease, offering researchers a reliable way to study molecular pathways involved in cellular metabolism and potential therapeutic strategies for metabolic disorders and cancer.
The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Purification Method:
Affinity purification
Gene ID:
5562/5563
Clone Number:
ARC62278
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from C2C12 cells, using Phospho-AMPKa1-T183/AMPKa2-T172 Rabbit mAb (CABP1441) at 1:1000 dilution.C2C12 cells were treated by mHGF(50ng/uL).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
