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Phospho-AKT2 specific-S474 Polyclonal Antibody (CABP1403)

SKU:
CABP1403
$167
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Description

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Product Name:Phospho-AKT2 specific-S474 Polyclonal Antibody
SKU:CABP1403
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:A synthetic phosphorylated peptide around S474 of human AKT2 specific .
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:PKBB; PRKBB; HIHGHH; PKBBETA; RAC-BETA; Phospho-AKT2 specific-S474
Positive Sample:293F treated by Calyculin A,293F treated by Calyculin A after transfected with AKT2
Conjugate:Unconjugated
Cellular Localization:Cell membrane, Cytoplasm, Early endosome, Nucleus, Peripheral membrane protein.
Calculated MW:56kDa
Observed MW:63kDa/90kDa

This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains, which is involved in signaling pathways. The gene serves as an oncogene in the tumorigenesis of cancer cells For example, its overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins, and has also been implicated in insulin signaling.

Purification Method:Affinity purification
Gene ID:208
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from 293F cells using Phospho-AKT2 specific-S474 Rabbit pAb (CABP1403) at 1:900 dilution. 293F cells were treated by Calyculin A (50 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

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