The Phospho-Akt2 Specific S474 Polyclonal Antibody is a valuable tool for researchers studying the Akt2 signaling pathway, a key regulator of cell growth and survival. This antibody, produced in rabbits, is highly specific to the phosphorylated form of Akt2 at serine 474, enabling precise detection and quantification in Western blot applications.Akt2 is a member of the Akt family of serine/threonine protein kinases, known for their role in promoting cell proliferation and inhibiting apoptosis. Phosphorylation of Akt2 at serine 474 is crucial for its activation and downstream signaling, making this antibody essential for studying the regulation of this important pathway.
With its high specificity and sensitivity, the Phospho-Akt2 Specific S474 Polyclonal Antibody is ideal for research in cancer biology, diabetes, and other diseases where Akt2 dysregulation plays a key role. By understanding the phosphorylation status of Akt2, researchers can gain valuable insights into the mechanisms underlying disease progression and potentially identify new therapeutic targets for intervention.
Product Name:
Phospho-AKT2 specific-S474 Polyclonal Antibody
SKU:
CABP1403
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around S474 of human AKT2 specific .
293F treated by Calyculin A,293F treated by Calyculin A after transfected with AKT2
Conjugate:
Unconjugated
Cellular Localization:
Cell membrane, Cytoplasm, Early endosome, Nucleus, Peripheral membrane protein.
Calculated MW:
56kDa
Observed MW:
63kDa/90kDa
This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains, which is involved in signaling pathways. The gene serves as an oncogene in the tumorigenesis of cancer cells For example, its overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins, and has also been implicated in insulin signaling.
Purification Method:
Affinity purification
Gene ID:
208
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from 293F cells using Phospho-AKT2 specific-S474 Rabbit pAb (CABP1403) at 1:900 dilution. 293F cells were treated by Calyculin A (50 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
