The Phospho-AKT2 specific-S474 Polyclonal Antibody (CABP1403) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains, which is involved in signaling pathways. The gene serves as an oncogene in the tumorigenesis of cancer cells For example, its overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins, and has also been implicated in insulin signaling. RRID Gene ID 208 Swiss Prot Synonym PKBB; PRKBB; HIHGHH; PKBBETA; RAC-BETA; Phospho-AKT2 specific-S474
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-AKT2 specific-S474 Polyclonal Antibody
SKU:
CABP1403
Size:
100μL, 20μL
Reactivity:
Human
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
293F treated with Calyculin A, 293F treated with Calyculin A after transfected with AKT2
Cellular Localization:
Cell Membrane, Cytoplasm, Early Endosome, Nucleus, Peripheral Membrane Protein.
Calculated MW:
56kDa
Observed MW:
63kDa/90kDa
This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains, which is involved in signaling pathways. The gene serves as an oncogene in the tumorigenesis of cancer cells For example, its overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins, and has also been implicated in insulin signaling. RRID Gene ID 208 Swiss Prot Synonym PKBB; PRKBB; HIHGHH; PKBBETA; RAC-BETA; Phospho-AKT2 specific-S474
Purification Method:
Affinity purification
Gene ID:
208
RRID:
-
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from 293F cells using Phospho-AKT2 specific-S474 Rabbit pAb (CABP1403) at 1:900 dilution. 293F cells were treated with Calyculin A (50 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Western blot analysis of lysates from 293F cells using Phospho-AKT2 specific-S474 Rabbit pAb (CABP1403) at 1:900 dilution. 293F cells,293F cells transfected with AKT3 and 293F cells transfected with AKT2 were treated with Calyculin A (50 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
