The Phospho-Akt2 (S474) Polyclonal Antibody (CABP0005) is an essential tool for researchers studying the Akt2 signaling pathway. This antibody, generated in rabbits, specifically targets the phosphorylated form of Akt2 at serine 474, a key regulatory site involved in cell survival and proliferation. It is validated for use in Western blot and immunohistochemistry applications, allowing for precise detection and analysis of Akt2 activation in various cell types and tissues.Akt2, a member of the protein kinase B (PKB) family, plays a crucial role in cell growth, metabolism, and survival through its regulation of multiple downstream signaling pathways. Phosphorylation of Akt2 at serine 474 enhances its kinase activity, promoting cell proliferation and survival in response to growth factors and other stimuli.
Dysregulation of Akt2 signaling has been implicated in various diseases, including cancer, diabetes, and cardiovascular disorders, making it a promising target for therapeutic intervention.By using the Phospho-Akt2 (S474) Polyclonal Antibody, researchers can gain insights into the activation status of Akt2 and its downstream targets in physiological and pathological conditions. This antibody is a valuable tool for investigating the molecular mechanisms underlying Akt2 signaling and identifying potential drug targets for the development of novel therapies.
Product Name:
Phospho-AKT2-S474 Rabbit Polyclonal Antibody
SKU:
CABP0005
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S474 of human AKT2 (NP_001617.1).
Cell membrane, Cytoplasm, Early endosome, Nucleus, Peripheral membrane protein.
Calculated MW:
56kDa
Observed MW:
60kDa
This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains, which is involved in signaling pathways. The gene serves as an oncogene in the tumorigenesis of cancer cells For example, its overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins, and has also been implicated in insulin signaling.
Purification Method:
Affinity purification
Gene ID:
208
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from 293 cells, using Phospho-AKT2-S474 Rabbit pAb (CABP0005) 1:1000 dilution..Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.
