The Phospho-Akt1S1 (T246) Polyclonal Antibody (CABP0793) is specifically designed for research involving the phosphorylation of Akt1S1 at threonine 246. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. It binds specifically to the phosphorylated form of Akt1S1, allowing for the detection and analysis of this important signaling molecule in various cell types.Akt1S1, a key component of the Akt signaling pathway, plays a crucial role in cell growth, proliferation, and survival. Phosphorylation of Akt1S1 at threonine 246 is known to regulate its activity and downstream signaling, making it a critical target for research in cancer, diabetes, and other diseases where Akt signaling is dysregulated.
By studying the phosphorylation status of Akt1S1 at threonine 246, researchers can gain valuable insights into the signaling cascades involved in cell growth and survival, and potentially identify new therapeutic targets for diseases where Akt signaling plays a role. The Phospho-Akt1S1 (T246) Polyclonal Antibody (CABP0793) is a valuable tool for researchers looking to explore the role of Akt1S1 phosphorylation in various biological processes.
A synthetic phosphorylated peptide around T246 of human AKT1S1/PRAS40 (NP_001092102.1).
Sequence:
LNTS D
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
Lobe; PRAS40; Phospho-AKT1S1/PRAS40-T246
Positive Sample:
HeLa
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, cytosol.
Calculated MW:
27kDa
Observed MW:
40kDa
AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).
Purification Method:
Affinity purification
Gene ID:
84335
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of HeLa, using Phospho-AKT1S1/PRAS40-T246 Rabbit pAb (CABP0793) at 1:400 dilution.HeLa cells were treated by Insulin (100 nM) at 37℃ for 5 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 45s.
