The Phospho-Akt1 (Y315)/Akt2 (Y316)/Akt3 (Y312) Polyclonal Antibody (CABP0274) is a valuable tool for researchers studying the Akt signaling pathway. Akt, also known as protein kinase B, plays a key role in cell survival, growth, and proliferation, making it a crucial target for cancer and metabolic disorder research. This antibody, raised in rabbits, is highly specific and reactive with human samples, allowing for accurate detection and analysis in Western blot experiments.The phosphorylation of Akt at specific tyrosine residues, including Y315 in Akt1, Y316 in Akt2, and Y312 in Akt3, is essential for its activation and downstream signaling. The Phospho-Akt1/Akt2/Akt3 Polyclonal Antibody enables researchers to study the activation status of Akt in various cell types and conditions, providing insights into its role in cellular processes such as apoptosis, metabolism, and proliferation.
By understanding the phosphorylation status of Akt isoforms, researchers can gain valuable insights into the mechanisms underlying cell growth and survival, as well as identify potential therapeutic targets for cancer and other diseases. The Phospho-Akt1/Akt2/Akt3 Polyclonal Antibody is a reliable tool for investigating Akt signaling dynamics and its implications in physiological and pathological processes.
A phospho specific peptide corresponding to residues surrounding Y315 of human AKT1
Purification Method:
Affinity purification
Storage Buffer:
Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:
IgG
Sequence:
Email for sequence
Gene ID:
207/208/10000
Uniprot:
P31749/P31751/Q9Y243
Cellular Location:
Calculated MW:
48kDa/55kDa/51kDa/54kDa
Observed MW:
56kDa
Western blot analysis of extracts HepG2 cells using Phospho-AKT1-Y315/AKT2-Y316/AKT3-Y312 antibody (CABP0274).HepG2 cells were treated by EGF (100 ng/mL) at 37'C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.
Synonyms:
AKT1/AKT2/AKT3
Background:
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]
