The Phospho-Akt1 (T450) Rabbit Monoclonal Antibody (CABP0980) is a valuable tool for research studies focused on Akt1, a key signaling protein involved in cell survival, growth, and proliferation. This antibody, developed using rabbit monoclonal technology, exhibits high specificity and sensitivity for detecting phosphorylated Akt1 at threonine 450 residue. It is validated for use in various applications, including Western blotting, immunohistochemistry, and immunofluorescence.Akt1 is a critical mediator of the PI3K/Akt signaling pathway, which plays a central role in regulating cellular processes such as metabolism, proliferation, and survival. Phosphorylation of Akt1 at threonine 450 is known to enhance its kinase activity, leading to downstream signaling events that promote cell growth and inhibit apoptosis.
By targeting this phosphorylation site, researchers can gain insights into the activation and regulation of Akt1 in different cellular contexts.The Phospho-Akt1 (T450) Rabbit Monoclonal Antibody provides a reliable tool for studying the role of Akt1 signaling in various physiological and pathological conditions, including cancer, diabetes, and neurodegenerative diseases. Its specificity for the phosphorylated form of Akt1 makes it an excellent choice for investigating the activation status of this important signaling molecule in different experimental models. Researchers can use this antibody to explore the mechanisms underlying Akt1 function and its potential as a therapeutic target in disease
Product Name:
Phospho-AKT1-T450 Rabbit Monoclonal Antibody
SKU:
CABP0980
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
A synthetic phosphorylated peptide around T450 of human Akt1 (P31749).
This gene encodes one of the three members of the human AKT serine-threonine protein kinase family which are often referred to as protein kinase B alpha, beta, and gamma. These highly similar AKT proteins all have an N-terminal pleckstrin homology domain, a serine/threonine-specific kinase domain and a C-terminal regulatory domain. These proteins are phosphorylated by phosphoinositide 3-kinase (PI3K). AKT/PI3K forms a key component of many signalling pathways that involve the binding of membrane-bound ligands such as receptor tyrosine kinases, G-protein coupled receptors, and integrin-linked kinase. These AKT proteins therefore regulate a wide variety of cellular functions including cell proliferation, survival, metabolism, and angiogenesis in both normal and malignant cells. AKT proteins are recruited to the cell membrane by phosphatidylinositol 3,4,5-trisphosphate (PIP3) after phosphorylation of phosphatidylinositol 4,5-bisphosphate (PIP2) by PI3K. Subsequent phosphorylation of both threonine residue 308 and serine residue 473 is required for full activation of the AKT1 protein encoded by this gene. Phosphorylation of additional residues also occurs, for example, in response to insulin growth factor-1 and epidermal growth factor. Protein phosphatases act as negative regulators of AKT proteins by dephosphorylating AKT or PIP3. The PI3K/AKT signalling pathway is crucial for tumor cell survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating AKT1 which then phosphorylates and inactivates components of the apoptotic machinery. AKT proteins also participate in the mammalian target of rapamycin (mTOR) signalling pathway which controls the assembly of the eukaryotic translation initiation factor 4F (eIF4E) complex and this pathway, in addition to responding to extracellular signals from growth factors and cytokines, is disregulated in many cancers. Mutations in this gene are associated with multiple types of cancer and excessive tissue growth including Proteus syndrome and Cowden syndrome 6, and breast, colorectal, and ovarian cancers. Multiple alternatively spliced transcript variants have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
207
Clone Number:
ARC1524
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from C6 cells, using Phospho-AKT1-T450 Rabbit mAb (CABP0980) at 1:1000 dilution.C6 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour or treated by PDGF (50 ng/mL) at 37℃ for 30 minutes after serum-starvation overnightSecondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 3min.
