The Phospho-AKT1 (T308) Polyclonal Antibody (CABP0304) is a valuable tool for researchers studying the Akt signaling pathway and its role in cell growth, proliferation, and survival. This antibody specifically recognizes the phosphorylated form of AKT1 at threonine 308, a critical site for AKT activation and downstream signaling. Raised in rabbits, this antibody is highly specific for human samples and has been validated for use in Western blot applications.AKT1, also known as protein kinase B, is a key player in intracellular signaling pathways that regulate cell metabolism, growth, and survival. Phosphorylation of AKT1 at threonine 308 is a crucial step in its activation, leading to the phosphorylation of downstream targets involved in cell proliferation and survival.
Dysregulation of AKT1 signaling has been implicated in a variety of diseases, including cancer, diabetes, and neurological disorders.By using the Phospho-AKT1 (T308) Polyclonal Antibody, researchers can effectively detect and analyze the activated form of AKT1 in various cell types and tissues, providing insight into the mechanisms of AKT signaling in health and disease. This antibody is an essential tool for studies in cell biology, cancer research, and drug development targeting the AKT pathway.
Product Name:
Phospho-AKT1-T308 Polyclonal Antibody
SKU:
CABP0304
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around T308 of human Akt1 (NP_005154.2).
This gene encodes one of the three members of the human AKT serine-threonine protein kinase family which are often referred to as protein kinase B alpha, beta, and gamma. These highly similar AKT proteins all have an N-terminal pleckstrin homology domain, a serine/threonine-specific kinase domain and a C-terminal regulatory domain. These proteins are phosphorylated by phosphoinositide 3-kinase (PI3K). AKT/PI3K forms a key component of many signalling pathways that involve the binding of membrane-bound ligands such as receptor tyrosine kinases, G-protein coupled receptors, and integrin-linked kinase. These AKT proteins therefore regulate a wide variety of cellular functions including cell proliferation, survival, metabolism, and angiogenesis in both normal and malignant cells. AKT proteins are recruited to the cell membrane by phosphatidylinositol 3,4,5-trisphosphate (PIP3) after phosphorylation of phosphatidylinositol 4,5-bisphosphate (PIP2) by PI3K. Subsequent phosphorylation of both threonine residue 308 and serine residue 473 is required for full activation of the AKT1 protein encoded by this gene. Phosphorylation of additional residues also occurs, for example, in response to insulin growth factor-1 and epidermal growth factor. Protein phosphatases act as negative regulators of AKT proteins by dephosphorylating AKT or PIP3. The PI3K/AKT signalling pathway is crucial for tumor cell survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating AKT1 which then phosphorylates and inactivates components of the apoptotic machinery. AKT proteins also participate in the mammalian target of rapamycin (mTOR) signalling pathway which controls the assembly of the eukaryotic translation initiation factor 4F (eIF4E) complex and this pathway, in addition to responding to extracellular signals from growth factors and cytokines, is disregulated in many cancers. Mutations in this gene are associated with multiple types of cancer and excessive tissue growth including Proteus syndrome and Cowden syndrome 6, and breast, colorectal, and ovarian cancers. Multiple alternatively spliced transcript variants have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
207
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from NIH/3T3 cells using Phospho-AKT1-T308 Rabbit pAb (CABP0304) at 1:600 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection:ECL Basic Kit (AbGn00020).Exposuretime: 30s.
