null

Phospho-AKT1-S473 Rabbit Polyclonal Antibody (CABP0140)

SKU:
CABP0140
Product Type:
Antibody
Applications:
WB
IHC
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Death
  • Cell Death Antibodies 2 Anti-Phospho-AKT1-S473 Antibody CABP0140
  • Cell Death Antibodies 2 Anti-Phospho-AKT1-S473 Antibody CABP0140
  • Cell Death Antibodies 2 Anti-Phospho-AKT1-S473 Antibody CABP0140
  • Cell Death Antibodies 2 Anti-Phospho-AKT1-S473 Antibody CABP0140
€419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS

Phospho-AKT1-S473 Rabbit Polyclonal Antibody (CABP0140)

The Phospho-AKT1 (S473) Polyclonal Antibody (CABP0140) offered by Assay Genie is a valuable tool for researchers studying the phosphorylation of AKT1 at serine 473, a key event in the activation of the AKT signaling pathway. This antibody, raised in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By targeting the phosphorylated form of AKT1 at serine 473, this antibody enables researchers to accurately detect and analyze the activation status of AKT1 in various cellular processes.Activation of AKT1 is known to play a critical role in cell growth, proliferation, and survival, making it a central player in the regulation of various cellular functions.

The phosphorylation of AKT1 at serine 473 is particularly important as it has been linked to increased AKT kinase activity and downstream signaling cascades. By using the Phospho-AKT1 (S473) Polyclonal Antibody, researchers can gain insights into the activation status of AKT1 and its role in different physiological and pathological conditions.Overall, the Phospho-AKT1 (S473) Polyclonal Antibody is a valuable tool for researchers investigating the AKT signaling pathway in various research areas, including cancer biology, cell signaling, and drug development. Its high reactivity and specificity for phosphorylated AKT1 make it an essential reagent for studies aiming to understand the

Product Name:Phospho-AKT1-S473 Rabbit Polyclonal Antibody
SKU:CABP0140
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic phosphorylated peptide around S473 of human Akt1 (NP_005154.2).
Sequence:QFSY S
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:AKT; PKB; RAC; PRKBA; PKB-ALPHA; RAC-ALPHA; Phospho-AKT1-S473
Positive Sample:Jurkat+Serum-starvation,Jurkat+Calyculin A,C6+Serum-starvation,C6+Calyculin A
Conjugate:Unconjugated
Cellular Localization:Cell membrane, Cytoplasm, Nucleus.
Calculated MW:56kDa
Observed MW:60kDa

This gene encodes one of the three members of the human AKT serine-threonine protein kinase family which are often referred to as protein kinase B alpha, beta, and gamma. These highly similar AKT proteins all have an N-terminal pleckstrin homology domain, a serine/threonine-specific kinase domain and a C-terminal regulatory domain. These proteins are phosphorylated by phosphoinositide 3-kinase (PI3K). AKT/PI3K forms a key component of many signalling pathways that involve the binding of membrane-bound ligands such as receptor tyrosine kinases, G-protein coupled receptors, and integrin-linked kinase. These AKT proteins therefore regulate a wide variety of cellular functions including cell proliferation, survival, metabolism, and angiogenesis in both normal and malignant cells. AKT proteins are recruited to the cell membrane by phosphatidylinositol 3,4,5-trisphosphate (PIP3) after phosphorylation of phosphatidylinositol 4,5-bisphosphate (PIP2) by PI3K. Subsequent phosphorylation of both threonine residue 308 and serine residue 473 is required for full activation of the AKT1 protein encoded by this gene. Phosphorylation of additional residues also occurs, for example, in response to insulin growth factor-1 and epidermal growth factor. Protein phosphatases act as negative regulators of AKT proteins by dephosphorylating AKT or PIP3. The PI3K/AKT signalling pathway is crucial for tumor cell survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating AKT1 which then phosphorylates and inactivates components of the apoptotic machinery. AKT proteins also participate in the mammalian target of rapamycin (mTOR) signalling pathway which controls the assembly of the eukaryotic translation initiation factor 4F (eIF4E) complex and this pathway, in addition to responding to extracellular signals from growth factors and cytokines, is disregulated in many cancers. Mutations in this gene are associated with multiple types of cancer and excessive tissue growth including Proteus syndrome and Cowden syndrome 6, and breast, colorectal, and ovarian cancers. Multiple alternatively spliced transcript variants have been found for this gene.

Purification Method:Affinity purification
Gene ID:207
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from Jurka cells, using Phospho-AKT1-S473 Rabbit pAb (CABP0140) at 1:1000 dilution.Jurkat cells were treated by Serum-starvation overnight at 37℃.Jurkat cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

Related Products