The Phospho-AKT1 (S473) Polyclonal Antibody (CABP0098) is a valuable tool for researchers studying the AKT1 protein and its phosphorylation at serine 473, a critical regulatory site implicated in cell growth, survival, and metabolism. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications.AKT1 is a key signaling protein involved in various cellular processes, including cell proliferation, survival, and metabolism. Phosphorylation of AKT1 at serine 473 is known to activate its kinase activity, leading to downstream signaling pathways that regulate cell growth and survival.
Understanding the regulation of AKT1 phosphorylation is essential for investigating its role in diseases such as cancer, diabetes, and cardiovascular disorders.By using the Phospho-AKT1 (S473) Polyclonal Antibody, researchers can confidently detect and analyze phosphorylated AKT1 in different cell types, providing valuable insights into its functions and signaling pathways. This antibody is an essential tool for studies in cell biology, cancer research, and drug development aimed at targeting the AKT signaling pathway for therapeutic interventions.
Product Name:
Phospho-AKT1-S473 Rabbit Polyclonal Antibody
SKU:
CABP0098
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S473 of human Akt1 (NP_005154.2).
This gene encodes one of the three members of the human AKT serine-threonine protein kinase family which are often referred to as protein kinase B alpha, beta, and gamma. These highly similar AKT proteins all have an N-terminal pleckstrin homology domain, a serine/threonine-specific kinase domain and a C-terminal regulatory domain. These proteins are phosphorylated by phosphoinositide 3-kinase (PI3K). AKT/PI3K forms a key component of many signalling pathways that involve the binding of membrane-bound ligands such as receptor tyrosine kinases, G-protein coupled receptors, and integrin-linked kinase. These AKT proteins therefore regulate a wide variety of cellular functions including cell proliferation, survival, metabolism, and angiogenesis in both normal and malignant cells. AKT proteins are recruited to the cell membrane by phosphatidylinositol 3,4,5-trisphosphate (PIP3) after phosphorylation of phosphatidylinositol 4,5-bisphosphate (PIP2) by PI3K. Subsequent phosphorylation of both threonine residue 308 and serine residue 473 is required for full activation of the AKT1 protein encoded by this gene. Phosphorylation of additional residues also occurs, for example, in response to insulin growth factor-1 and epidermal growth factor. Protein phosphatases act as negative regulators of AKT proteins by dephosphorylating AKT or PIP3. The PI3K/AKT signalling pathway is crucial for tumor cell survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating AKT1 which then phosphorylates and inactivates components of the apoptotic machinery. AKT proteins also participate in the mammalian target of rapamycin (mTOR) signalling pathway which controls the assembly of the eukaryotic translation initiation factor 4F (eIF4E) complex and this pathway, in addition to responding to extracellular signals from growth factors and cytokines, is disregulated in many cancers. Mutations in this gene are associated with multiple types of cancer and excessive tissue growth including Proteus syndrome and Cowden syndrome 6, and breast, colorectal, and ovarian cancers. Multiple alternatively spliced transcript variants have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
207
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from C6 cells using Phospho-AKT1-S473 Rabbit pAb (CABP0098) at 1:400 dilution. C6 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
