The Phospho-Akt1 (S473) / Akt2 (S474) / Akt3 (S472) Rabbit Polyclonal Antibody (CABP1068) is a valuable tool for researchers studying the Akt pathway, a key signaling pathway involved in cell survival, proliferation, and metabolism. This antibody, raised in rabbits, is highly specific and sensitive, allowing for the detection and analysis of phosphorylated Akt isoforms in various cell types.Phosphorylation of Akt at serine residues 473 (Akt1) and 474 (Akt2), as well as threonine residue 472 (Akt3), is crucial for Akt activation and downstream signaling. Dysregulation of the Akt pathway has been implicated in various diseases, including cancer, diabetes, and neurological disorders.
The CABP1068 antibody enables researchers to study the phosphorylation status of Akt isoforms, providing insights into their role in disease pathogenesis and therapeutic targeting.By using the Phospho-Akt1 (S473) / Akt2 (S474) / Akt3 (S472) Rabbit Polyclonal Antibody (CABP1068), researchers can advance their understanding of the Akt pathway and its implications for human health. This antibody is suitable for Western blot applications and is validated for use with human samples, making it a valuable tool for studies in cancer biology, metabolic disorders, and neurodegenerative diseases.
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]
Purification Method:
Affinity purification
Gene ID:
207/208/10000
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from C6 cells using Phospho-Akt-S473 Rabbit pAb (CABP1068) at 1:500 dilution. C6 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
