Phospho-AKT1-S473+AKT2-S474+AKT3-S472 Rabbit Polyclonal Antibody (CABP1068)
- SKU:
- CABP1068
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
Product Name: | Phospho-AKT1-S473+AKT2-S474+AKT3-S472 Rabbit Polyclonal Antibody |
SKU: | CABP1068 |
Size: | 20uL, 100uL |
Isotype: | IgG |
Host Species: | Rabbit |
Reactivity: | Human,Mouse,Rat |
Immunogen: | A synthetic phosphorylated peptide around S473 of human AKT1/AKT2/AKT3AKT1 (NP_005154.2). |
Sequence: | QFSY S |
Tested Applications: | WB IHC-P ELISA |
Recommended Dilution: | WB,1:500 - 1:2000 IHC-P,1:50 - 1:200 |
Synonyms: | AKT1/AKT2/AKT3; Phospho-Akt-S473 |
Positive Sample: | NIH/3T3,C6,Jurkat |
Cellular Localization: | ciliary basal body, cytoplasm, cytosol, microtubule cytoskeleton, mitochondrion, nucleoplasm, nucleus, plasma membrane, spindle |
Calculated MW: | 48kDa/55kDa/51kDa/54kDa |
Observed MW: | 60kDa |
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]
Purification Method: | Affinity purification |
Gene ID: | 207/208/10000 |
Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |