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Phospho-AKT1-S129 Rabbit Monoclonal Antibody (CABP0981)

SKU:
CABP0981
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Monoclonal Antibody
Research Area:
Cell Death
€419
Frequently bought together:

Description

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Phospho-AKT1-S129 Rabbit Monoclonal Antibody (CABP0981)

The Phospho-Akt1 (S129) Rabbit Monoclonal Antibody (CABP0981) is specifically designed for researchers studying the Akt1 protein, a key player in cell survival and proliferation pathways. This antibody, generated in rabbits, is highly specific and sensitive for detecting phosphorylated Akt1 at serine 129. Phosphorylation of Akt1 at S129 has been linked to various cellular processes, including cell growth, proliferation, and survival. The antibody has been validated for use in Western blot applications, allowing for the detection and analysis of phosphorylated Akt1 in various cell types.

Akt1, a member of the protein kinase B (PKB) family, is involved in multiple signaling pathways and is dysregulated in various diseases, including cancer and diabetes. Understanding the phosphorylation status of Akt1 at S129 can provide valuable insights into its activation and function in these pathological conditions.Overall, the Phospho-Akt1 (S129) Rabbit Monoclonal Antibody (CABP0981) is a powerful tool for researchers interested in studying the role of Akt1 phosphorylation in cell signaling pathways and its implications in disease development and progression.

Product Name:Phospho-AKT1-S129 Rabbit Monoclonal Antibody
SKU:CABP0981
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:A synthetic phosphorylated peptide around S129 of human Akt1 (P31749).
Sequence:DNSG A
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Synonyms:AKT; PKB; RAC; PRKBA; PKB-ALPHA; RAC-ALPHA; Phospho-AKT1-S129
Positive Sample:MCF7
Conjugate:Unconjugated
Cellular Localization:Cell membrane, Cytoplasm, Nucleus.
Calculated MW:56kDa
Observed MW:60kDa

This gene encodes one of the three members of the human AKT serine-threonine protein kinase family which are often referred to as protein kinase B alpha, beta, and gamma. These highly similar AKT proteins all have an N-terminal pleckstrin homology domain, a serine/threonine-specific kinase domain and a C-terminal regulatory domain. These proteins are phosphorylated by phosphoinositide 3-kinase (PI3K). AKT/PI3K forms a key component of many signalling pathways that involve the binding of membrane-bound ligands such as receptor tyrosine kinases, G-protein coupled receptors, and integrin-linked kinase. These AKT proteins therefore regulate a wide variety of cellular functions including cell proliferation, survival, metabolism, and angiogenesis in both normal and malignant cells. AKT proteins are recruited to the cell membrane by phosphatidylinositol 3,4,5-trisphosphate (PIP3) after phosphorylation of phosphatidylinositol 4,5-bisphosphate (PIP2) by PI3K. Subsequent phosphorylation of both threonine residue 308 and serine residue 473 is required for full activation of the AKT1 protein encoded by this gene. Phosphorylation of additional residues also occurs, for example, in response to insulin growth factor-1 and epidermal growth factor. Protein phosphatases act as negative regulators of AKT proteins by dephosphorylating AKT or PIP3. The PI3K/AKT signalling pathway is crucial for tumor cell survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating AKT1 which then phosphorylates and inactivates components of the apoptotic machinery. AKT proteins also participate in the mammalian target of rapamycin (mTOR) signalling pathway which controls the assembly of the eukaryotic translation initiation factor 4F (eIF4E) complex and this pathway, in addition to responding to extracellular signals from growth factors and cytokines, is disregulated in many cancers. Mutations in this gene are associated with multiple types of cancer and excessive tissue growth including Proteus syndrome and Cowden syndrome 6, and breast, colorectal, and ovarian cancers. Multiple alternatively spliced transcript variants have been found for this gene.

Purification Method:Affinity purification
Gene ID:207
Clone Number:ARC1525
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of MCF7 cells, using Phospho-AKT1-S129 Rabbit mAb (CABP0981) at 1:1000 dilution.MCF7 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.

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