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Phospho-AKT1-S129 Polyclonal Antibody (CABP1272)

SKU:
CABP1272
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
€179 - €469
Frequently bought together:

Description

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Phospho-AKT1-S129 Polyclonal Antibody (CABP1272)

The Phospho-AKT1 (S129) Polyclonal Antibody (CABP1272) is specifically designed for research involving the phosphorylation of AKT1 at serine 129, a key post-translational modification that regulates the activity of this critical signaling molecule. The antibody, produced in rabbits, is highly specific and sensitive for detecting phosphorylated AKT1 in human samples, making it an invaluable tool for studying signal transduction pathways in various biological systems.AKT1 is a serine/threonine protein kinase that plays a crucial role in cell survival, proliferation, and metabolism. Phosphorylation of AKT1 at serine 129 has been implicated in the regulation of its activity, affecting downstream signaling cascades involved in cell growth, apoptosis, and nutrient metabolism.

The ability to detect and quantify phosphorylated AKT1 in different cellular contexts provides valuable insights into the mechanisms governing cell signaling and may uncover new therapeutic targets for diseases such as cancer and metabolic disorders.Overall, the Phospho-AKT1 (S129) Polyclonal Antibody (CABP1272) is a reliable reagent for researchers interested in unraveling the complexities of AKT1 signaling and its role in various physiological and pathological processes. Its high specificity and sensitivity make it an essential tool for elucidating the functional significance of AKT1 phosphorylation and its potential implications for human health.

Product Name:Phospho-AKT1-S129 Polyclonal Antibody
SKU:CABP1272
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:A synthetic phosphorylated peptide around S129 of human Akt1 (NP_005154.2).
Sequence:DNSG A
Tested Applications:WB ELISA
Recommended Dilution:WB,1:100 - 1:500
Synonyms:AKT; PKB; RAC; PRKBA; PKB-ALPHA; RAC-ALPHA; Phospho-AKT1-S129
Positive Sample:293T
Conjugate:Unconjugated
Cellular Localization:Cell membrane, Cytoplasm, Nucleus.
Calculated MW:56kDa
Observed MW:60kDa

This gene encodes one of the three members of the human AKT serine-threonine protein kinase family which are often referred to as protein kinase B alpha, beta, and gamma. These highly similar AKT proteins all have an N-terminal pleckstrin homology domain, a serine/threonine-specific kinase domain and a C-terminal regulatory domain. These proteins are phosphorylated by phosphoinositide 3-kinase (PI3K). AKT/PI3K forms a key component of many signalling pathways that involve the binding of membrane-bound ligands such as receptor tyrosine kinases, G-protein coupled receptors, and integrin-linked kinase. These AKT proteins therefore regulate a wide variety of cellular functions including cell proliferation, survival, metabolism, and angiogenesis in both normal and malignant cells. AKT proteins are recruited to the cell membrane by phosphatidylinositol 3,4,5-trisphosphate (PIP3) after phosphorylation of phosphatidylinositol 4,5-bisphosphate (PIP2) by PI3K. Subsequent phosphorylation of both threonine residue 308 and serine residue 473 is required for full activation of the AKT1 protein encoded by this gene. Phosphorylation of additional residues also occurs, for example, in response to insulin growth factor-1 and epidermal growth factor. Protein phosphatases act as negative regulators of AKT proteins by dephosphorylating AKT or PIP3. The PI3K/AKT signalling pathway is crucial for tumor cell survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating AKT1 which then phosphorylates and inactivates components of the apoptotic machinery. AKT proteins also participate in the mammalian target of rapamycin (mTOR) signalling pathway which controls the assembly of the eukaryotic translation initiation factor 4F (eIF4E) complex and this pathway, in addition to responding to extracellular signals from growth factors and cytokines, is disregulated in many cancers. Mutations in this gene are associated with multiple types of cancer and excessive tissue growth including Proteus syndrome and Cowden syndrome 6, and breast, colorectal, and ovarian cancers. Multiple alternatively spliced transcript variants have been found for this gene.

Purification Method:Affinity purification
Gene ID:207
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of 293T cells, using Phospho-AKT1-S129 antibody (CABP1272) at 1:500 dilution.293T cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.

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