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Phospho-AKT1-S124 Rabbit Monoclonal Antibody (CABP0982)

SKU:
CABP0982
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Monoclonal Antibody
Research Area:
Cell Death
€419
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Description

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Phospho-AKT1-S124 Rabbit Monoclonal Antibody (CABP0982)

The Phospho-Akt1 (S124) Rabbit Monoclonal Antibody (CABP0982) is a crucial tool for researchers studying the Akt1 signaling pathway, which plays a key role in cell survival and growth. This antibody specifically targets the phosphorylated form of Akt1 at serine 124, allowing for precise detection and analysis of this important regulatory protein.With high reactivity towards human samples, the Phospho-Akt1 (S124) Rabbit Monoclonal Antibody is ideal for use in Western blot applications. By targeting the phosphorylated form of Akt1, researchers can gain insights into the activation status of this critical signaling molecule in various cell types.

Akt1 dysregulation is implicated in numerous diseases, including cancer, diabetes, and cardiovascular disorders. Understanding the phosphorylation status of Akt1 at serine 124 can provide valuable information for studies in these fields and guide the development of targeted therapeutics.Overall, the Phospho-Akt1 (S124) Rabbit Monoclonal Antibody (CABP0982) is a reliable tool for investigating the Akt1 signaling pathway and its role in health and disease. Its specificity and sensitivity make it a valuable asset for researchers in the fields of cell biology, oncology, and drug development.

Product Name:Phospho-AKT1-S124 Rabbit Monoclonal Antibody
SKU:CABP0982
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:A synthetic phosphorylated peptide around S124 of human Akt1 (P31749).
Sequence:SGSP S
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Synonyms:AKT; PKB; RAC; PRKBA; PKB-ALPHA; RAC-ALPHA; Phospho-AKT1-S124
Positive Sample:MCF7
Conjugate:Unconjugated
Cellular Localization:Cell membrane, Cytoplasm, Nucleus.
Calculated MW:56kDa
Observed MW:60kDa

This gene encodes one of the three members of the human AKT serine-threonine protein kinase family which are often referred to as protein kinase B alpha, beta, and gamma. These highly similar AKT proteins all have an N-terminal pleckstrin homology domain, a serine/threonine-specific kinase domain and a C-terminal regulatory domain. These proteins are phosphorylated by phosphoinositide 3-kinase (PI3K). AKT/PI3K forms a key component of many signalling pathways that involve the binding of membrane-bound ligands such as receptor tyrosine kinases, G-protein coupled receptors, and integrin-linked kinase. These AKT proteins therefore regulate a wide variety of cellular functions including cell proliferation, survival, metabolism, and angiogenesis in both normal and malignant cells. AKT proteins are recruited to the cell membrane by phosphatidylinositol 3,4,5-trisphosphate (PIP3) after phosphorylation of phosphatidylinositol 4,5-bisphosphate (PIP2) by PI3K. Subsequent phosphorylation of both threonine residue 308 and serine residue 473 is required for full activation of the AKT1 protein encoded by this gene. Phosphorylation of additional residues also occurs, for example, in response to insulin growth factor-1 and epidermal growth factor. Protein phosphatases act as negative regulators of AKT proteins by dephosphorylating AKT or PIP3. The PI3K/AKT signalling pathway is crucial for tumor cell survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating AKT1 which then phosphorylates and inactivates components of the apoptotic machinery. AKT proteins also participate in the mammalian target of rapamycin (mTOR) signalling pathway which controls the assembly of the eukaryotic translation initiation factor 4F (eIF4E) complex and this pathway, in addition to responding to extracellular signals from growth factors and cytokines, is disregulated in many cancers. Mutations in this gene are associated with multiple types of cancer and excessive tissue growth including Proteus syndrome and Cowden syndrome 6, and breast, colorectal, and ovarian cancers. Multiple alternatively spliced transcript variants have been found for this gene.

Purification Method:Affinity purification
Gene ID:207
Clone Number:ARC1526
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of MCF7 cells, using Phospho-AKT1-S124 Rabbit mAb (CABP0982) at 1:1000 dilution.MCF7 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.

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