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Phospho-Akt-T308 Monoclonal Antibody (CABP1332)

SKU:
CABP1332
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Applications:
WB
IHC
$167 - $731
Frequently bought together:

Description

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Phospho-Akt-T308 Monoclonal Antibody (CABP1332)

The Phospho-Akt (T308) Monoclonal Antibody is a valuable tool for researchers studying the Akt signaling pathway, which plays a crucial role in cell growth, survival, and metabolism. This antibody, developed for use in Western blot applications, specifically targets the phosphorylated form of Akt at threonine 308, a key regulatory site that activates Akt kinase activity.By binding to the phosphorylated Akt protein, this antibody allows for the detection and quantification of Akt activation in various cell types and experimental conditions.

This makes it an essential tool for studies in cancer research, where dysregulation of the Akt pathway is commonly observed.The Phospho-Akt (T308) Monoclonal Antibody is highly specific for human samples and has been rigorously validated to ensure reliable and reproducible results. Its use in research can provide valuable insights into the role of Akt signaling in disease development and progression, potentially leading to the identification of novel therapeutic targets for cancer and other related conditions.

Product Name:Phospho-Akt-T308 Monoclonal Antibody
SKU:CABP1332
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic phosphorylated peptide around T308 of human Phospho-Akt-T308 (NP_005154.2).
Sequence:MKTF C
Tested Applications:WB IHC-P ELISA
Recommended Dilution:WB,1:1000 - 1:5000 IHC-P,1:50 - 1:200
Synonyms:AKT; PKB; RAC; PRKBA; PKB-ALPHA; RAC-ALPHA; Phospho-Akt-T308
Positive Sample:Jurkat（grown in serum free media overnight, then treated by Calyculin A (100 nM) at 37℃ for 30 minutes）whole cell lysate
Conjugate:Unconjugated
Cellular Localization:Cell membrane, Cytoplasm, Nucleus.
Calculated MW:56kDa
Observed MW:65kDa

This gene encodes one of the three members of the human AKT serine-threonine protein kinase family which are often referred to as protein kinase B alpha, beta, and gamma. These highly similar AKT proteins all have an N-terminal pleckstrin homology domain, a serine/threonine-specific kinase domain and a C-terminal regulatory domain. These proteins are phosphorylated by phosphoinositide 3-kinase (PI3K). AKT/PI3K forms a key component of many signalling pathways that involve the binding of membrane-bound ligands such as receptor tyrosine kinases, G-protein coupled receptors, and integrin-linked kinase. These AKT proteins therefore regulate a wide variety of cellular functions including cell proliferation, survival, metabolism, and angiogenesis in both normal and malignant cells. AKT proteins are recruited to the cell membrane by phosphatidylinositol 3,4,5-trisphosphate (PIP3) after phosphorylation of phosphatidylinositol 4,5-bisphosphate (PIP2) by PI3K. Subsequent phosphorylation of both threonine residue 308 and serine residue 473 is required for full activation of the AKT1 protein encoded by this gene. Phosphorylation of additional residues also occurs, for example, in response to insulin growth factor-1 and epidermal growth factor. Protein phosphatases act as negative regulators of AKT proteins by dephosphorylating AKT or PIP3. The PI3K/AKT signalling pathway is crucial for tumor cell survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating AKT1 which then phosphorylates and inactivates components of the apoptotic machinery. AKT proteins also participate in the mammalian target of rapamycin (mTOR) signalling pathway which controls the assembly of the eukaryotic translation initiation factor 4F (eIF4E) complex and this pathway, in addition to responding to extracellular signals from growth factors and cytokines, is disregulated in many cancers. Mutations in this gene are associated with multiple types of cancer and excessive tissue growth including Proteus syndrome and Cowden syndrome 6, and breast, colorectal, and ovarian cancers. Multiple alternatively spliced transcript variants have been found for this gene.

Purification Method:Affinity purification
Gene ID:207
Clone Number:ARC55284
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from Jurkat cells, using Phospho-Akt-T308 Rabbit mAb (CABP1332) at1:2000 dilution.Jurkat cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.

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