The Phospho-ACLY (S455) Polyclonal Antibody (CABP0779) is a valuable tool for researchers studying ACLY phosphorylation at serine 455. ACLY is a key enzyme involved in fatty acid synthesis and is regulated by phosphorylation at various sites, including S455. This antibody, raised in rabbits, exhibits high specificity for human samples and is validated for use in Western blot applications.Phosphorylation of ACLY at S455 has been implicated in various cellular processes, including lipid metabolism and cell proliferation. This antibody enables the detection and analysis of phosphorylated ACLY in different cell types, making it suitable for research in metabolism, cell signaling, and cancer biology.
The ability to study ACLY phosphorylation at S455 can provide valuable insights into the regulation of lipid metabolism and cellular growth. Researchers can use this antibody to further understand the role of ACLY in physiological and pathological conditions, potentially leading to the development of novel therapeutic strategies for metabolic disorders and cancer.
Product Name:
Phospho-ACLY-S455 Rabbit Polyclonal Antibody
SKU:
CABP0779
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S455 of human ACLY (NP_001087.2).
Sequence:
RTAS FS
Tested Applications:
WBIHC-PIPELISA
Recommended Dilution:
WB,1:100 - 1:500 IHC-P,1:50 - 1:100 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
ACL; ATPCL; CLATP; Phospho-ACLY-S455
Positive Sample:
C6,NIH/3T3,HeLa
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm.
Calculated MW:
121kDa
Observed MW:
125kDa
ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene.
Purification Method:
Affinity purification
Gene ID:
47
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from C6 cells using Phospho-ACLY-S455 Rabbit pAb (CABP0779) at 1:400 dilution. C6 cells were treated by Insulin (100 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
