Phospho-Acetyl CoA Carboxylase-S79 Polyclonal Antibody (CABP1410)
- SKU:
- CABP1410
- Product Type:
- Antibody
- Antibody Type:
- Polyclonal Antibody
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- WB
Frequently bought together:
Description
| Product Name: | Phospho-Acetyl CoA Carboxylase-S79 Polyclonal Antibody |
| SKU: | CABP1410 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Mouse |
| Immunogen: | A synthetic phosphorylated peptide around S79 of human Acetyl CoA Carboxylase. |
| Tested Applications: | WB ELISA |
| Recommended Dilution: | WB,1:500 - 1:2000 |
| Synonyms: | ACC; ACAC; ACC1; ACCA; Acac1; hACC1; ACACAD; ACCalpha; ACACalpha; Phospho-Acetyl CoA Carboxylase-S79 |
| Positive Sample: | NIH/3T3 |
| Conjugate: | Unconjugated |
| Calculated MW: | 265kDa |
| Observed MW: | 280kDa |
Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene.
| Purification Method: | Affinity purification |
| Gene ID: | 31/32 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3. |
 | Western blot analysis of NIH/3T3, using Phospho-Acetyl CoA Carboxylase-S79 Rabbit PAb (CABP1410) at 1:1000 dilution.NIH/3T3 cells were treated by oligomycin (0.5 uM) at 37℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s. |
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