The Phospho-ABL1 (Y412) Rabbit Polyclonal Antibody (CABP1060) is a valuable tool for researchers studying ABL1, a tyrosine kinase involved in cell growth and survival. This antibody, raised in rabbits, specifically detects the phosphorylated form of ABL1 at tyrosine 412 and has been validated for use in Western blot applications. It enables the detection and analysis of phosphorylated ABL1 in human samples, providing insights into the regulation of cell signaling pathways.ABL1 is a key player in various cellular processes, including cell proliferation, differentiation, and migration.
Its aberrant activation has been linked to the development and progression of cancer, making it an attractive target for cancer therapy. By targeting the phosphorylated form of ABL1 at tyrosine 412, researchers can investigate its role in cancer progression and potentially identify novel therapeutic strategies.Overall, the Phospho-ABL1 (Y412) Rabbit Polyclonal Antibody (CABP1060) is a valuable tool for studying the signaling pathways involving ABL1 in cancer and other diseases, providing valuable insights for developing targeted therapies.
Product Name:
Phospho-ABL1-Y412 Rabbit Polyclonal Antibody
SKU:
CABP1060
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around Y412 of human ABL1 (NP_005148.2).
This gene is a protooncogene that encodes a protein tyrosine kinase involved in a variety of cellular processes, including cell division, adhesion, differentiation, and response to stress. The activity of the protein is negatively regulated by its SH3 domain, whereby deletion of the region encoding this domain results in an oncogene. The ubiquitously expressed protein has DNA-binding activity that is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function. This gene has been found fused to a variety of translocation partner genes in various leukemias, most notably the t(9;22) translocation that results in a fusion with the 5' end of the breakpoint cluster region gene (BCR; MIM:151410). Alternative splicing of this gene results in two transcript variants, which contain alternative first exons that are spliced to the remaining common exons.
Purification Method:
Affinity purification
Gene ID:
25
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from C6 cells, using Phospho-c-Abl-Y412 Rabbit pAb (CAB0282).C6 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
