The Phospho-ABL1 (Y245) Polyclonal Antibody (CABP0001) is a valuable tool for researchers studying the ABL1 protein, a tyrosine kinase involved in cell signaling and regulation. This antibody, generated in rabbits, is highly specific for the phosphorylated form of ABL1 at tyrosine 245 in human samples and is validated for use in Western blot applications. By targeting the phosphorylated form of ABL1, researchers can investigate signaling pathways and potential therapeutic targets in cancer and other diseases.ABL1 is a crucial player in cellular processes such as cell growth, differentiation, and survival, making it a key target for drug development in cancer treatment.
The phosphorylation of ABL1 at tyrosine 245 is known to regulate its activity and downstream signaling pathways, highlighting the importance of studying this specific modification. The Phospho-ABL1 (Y245) Polyclonal Antibody enables researchers to detect and quantify this phosphorylated form of ABL1 in various cell types, providing valuable insights into its function and potential as a therapeutic target in cancer research.
Product Name:
Phospho-ABL1-Y245 Rabbit Polyclonal Antibody
SKU:
CABP0001
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around Y245 of human ABL1 (NP_005148.2).
This gene is a protooncogene that encodes a protein tyrosine kinase involved in a variety of cellular processes, including cell division, adhesion, differentiation, and response to stress. The activity of the protein is negatively regulated by its SH3 domain, whereby deletion of the region encoding this domain results in an oncogene. The ubiquitously expressed protein has DNA-binding activity that is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function. This gene has been found fused to a variety of translocation partner genes in various leukemias, most notably the t(9;22) translocation that results in a fusion with the 5' end of the breakpoint cluster region gene (BCR; MIM:151410). Alternative splicing of this gene results in two transcript variants, which contain alternative first exons that are spliced to the remaining common exons.
Purification Method:
Affinity purification
Gene ID:
25
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from C6 cells, using Phospho-c-Abl-Y245 Rabbit pAb (CAB0282).C6 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3S.
