The PHKG2 Polyclonal Antibody (CAB7337) is a valuable tool for researchers studying PHKG2, a key enzyme involved in the regulation of glycogen metabolism and energy production. This antibody, raised in rabbits, exhibits high reactivity with human samples and is specifically validated for use in Western blot applications. By binding to the PHKG2 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an essential component for studies in biochemistry and metabolism research.PHKG2, also known as phosphorylase kinase gamma subunit, plays a crucial role in the phosphorylation of glycogen phosphorylase and the subsequent release of glucose for energy production in the body.
Dysregulation of PHKG2 activity has been linked to metabolic disorders such as glycogen storage diseases and diabetes, making it a promising target for therapeutic interventions. By investigating the function of PHKG2, researchers can gain valuable insights into the mechanisms underlying these diseases and develop potential treatment strategies to address them.
Product Name:
PHKG2 Rabbit Polyclonal Antibody
SKU:
CAB7337
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 237-406 of human PHKG2 (NP_000285.1).
Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The alpha subunit includes the skeletal muscle and hepatic isoforms, encoded by two different genes. The beta subunit is the same in both the muscle and hepatic isoforms, and encoded by one gene. The gamma subunit also includes the skeletal muscle and hepatic isoforms, and the hepatic isoform is encoded by this gene. The delta subunit is a calmodulin and can be encoded by three different genes. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. Mutations in this gene cause glycogen storage disease type 9C, also known as autosomal liver glycogenosis. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene.
Purification Method:
Affinity purification
Gene ID:
5261
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PHKG2 antibody (CAB7337) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
