The PHKB Polyclonal Antibody (CAB8015) is designed for researchers studying PHKB, a key enzyme involved in glycogen metabolism. The antibody, generated in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding to the PHKB protein, this antibody enables accurate detection and analysis in various cell types, making it a valuable tool for studies in metabolic disorders and glycogen storage diseases.PHKB is essential for the breakdown of glycogen into glucose for energy production in cells. Dysregulation of PHKB activity is associated with metabolic diseases such as glycogen storage disease type IX, where impaired glycogen metabolism leads to symptoms like muscle weakness and fatigue.
Research on PHKB function is crucial for understanding the molecular mechanisms underlying these disorders and may inform the development of targeted therapeutics in the future.Overall, the PHKB Polyclonal Antibody (CAB8015) offers researchers a reliable tool for investigating PHKB-related pathways and diseases, paving the way for advancements in glycogen metabolism research and potential therapeutic strategies.
Product Name:
PHKB Rabbit Polyclonal Antibody
SKU:
CAB8015
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 854-1093 of human PHKB (NP_000284.1).
Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The alpha subunit includes the skeletal muscle and hepatic isoforms, encoded by two different genes. The beta subunit is the same in both the muscle and hepatic isoforms, encoded by this gene, which is a member of the phosphorylase b kinase regulatory subunit family. The gamma subunit also includes the skeletal muscle and hepatic isoforms, encoded by two different genes. The delta subunit is a calmodulin and can be encoded by three different genes. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. Mutations in this gene cause glycogen storage disease type 9B, also known as phosphorylase kinase deficiency of liver and muscle. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene. Two pseudogenes have been found on chromosomes 14 and 20, respectively.
Purification Method:
Affinity purification
Gene ID:
5257
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PHKB antibody (CAB8015) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
