PHKB Rabbit Polyclonal Antibody (CAB8015)
- SKU:
- CAB8015
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Metabolism
Description
Product Name: | PHKB Rabbit Polyclonal Antibody |
SKU: | CAB8015 |
Size: | 20uL, 100uL |
Isotype: | IgG |
Host Species: | Rabbit |
Reactivity: | Human,Mouse |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 854-1093 of human PHKB (NP_000284.1). |
Sequence: | VIHI GWII SNNP ELFS GMLK IRIG WIIH AMEY ELQI RGGD KPAL DLYQ LSPS EVKQ LLLD ILQP QQNG RCWL NRRQ IDGS LNRT PTGF YDRV WQIL ERTP NGII VAGK HLPQ QPTL SDMT MYEM NFSL LVED TLGN IDQP QYRQ IVVE LLMV VSIV LERN PELE FQDK VDLD RLVK EAFN EFQK DQSR LKEI EKQD DMTS FYNT PPLG KRGT CSYL TKAV MNLL LEGE VKPN NDDP CLIS |
Tested Applications: | WB ELISA |
Recommended Dilution: | WB,1:500 - 1:2000 |
Synonyms: | PHKB |
Positive Sample: | HepG2,SW620,Jurkat,BT-474 |
Conjugate: | Unconjugated |
Cellular Localization: | Cell membrane, Cytoplasmic side, Lipid-anchor. |
Calculated MW: | 125kDa |
Observed MW: | 125kDa |
Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The alpha subunit includes the skeletal muscle and hepatic isoforms, encoded by two different genes. The beta subunit is the same in both the muscle and hepatic isoforms, encoded by this gene, which is a member of the phosphorylase b kinase regulatory subunit family. The gamma subunit also includes the skeletal muscle and hepatic isoforms, encoded by two different genes. The delta subunit is a calmodulin and can be encoded by three different genes. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. Mutations in this gene cause glycogen storage disease type 9B, also known as phosphorylase kinase deficiency of liver and muscle. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene. Two pseudogenes have been found on chromosomes 14 and 20, respectively.
Purification Method: | Affinity purification |
Gene ID: | 5257 |
Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. |